The College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, 450002, PR China.
The College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, 450002, PR China; Key Laboratory for Animal-derived Food Safety of Henan Province, Zhengzhou, Henan 450002, PR China.
Virus Res. 2020 Nov;289:198167. doi: 10.1016/j.virusres.2020.198167. Epub 2020 Sep 19.
Transmissible gastroenteritis virus (TGEV) and porcine deltacoronavirus (PDCoV) are members of the family coronaviridae and mainly cause acute diarrhea/vomiting, dehydration and mortality in piglets, which lead to huge economic losses to the swine industry. Rhodanine derivative LJ001 has been verified to be effective against some enveloped virus infections in vitro. In this study, we evaluated the antiviral activity of LJ001 towards TGEV and PDCoV replication on swine testicular(ST) cells. Our results showed the 50 % cellular cytotoxicity (CC) value of LJ001 was 146.4 μM on ST cell. The virus titers of TGEV and PDCoV were obviously decreased in the presence of LJ001 with the concentrations of 3.125 and 12.5 μM, and LJ001 potently inhibited TGEV and PDCoV infection at the replication stages of viral life cycle. Further study indicated that LJ001 inhibited TGEV and PDCoV replication by inhibition of viral RNA and protein synthesis, and reducing virus yields at 12 and 24 h post-inoculation. These data indicated that LJ001 had antiviral activities on TGEV and PDCoV replications in vitro, which may serve as a new candidate for treatment of coronaviruses infections.
传染性胃肠炎病毒（TGEV）和猪德尔塔冠状病毒（PDCoV）属于冠状病毒科，主要引起仔猪急性腹泻/呕吐、脱水和死亡，给养猪业造成巨大经济损失。硫代氨基脲衍生物 LJ001 已被证明在体外对某些包膜病毒感染有效。在本研究中，我们评估了 LJ001 对猪睾丸（ST）细胞中 TGEV 和 PDCoV 复制的抗病毒活性。结果表明，LJ001 在 ST 细胞中的 50%细胞毒性（CC）值为 146.4 μM。在 3.125 和 12.5 μM 浓度下，TGEV 和 PDCoV 的病毒滴度明显降低，LJ001 可在病毒生命周期的复制阶段有效抑制 TGEV 和 PDCoV 的感染。进一步的研究表明，LJ001 通过抑制病毒 RNA 和蛋白质合成，并在接种后 12 和 24 小时减少病毒产量，抑制 TGEV 和 PDCoV 的复制。这些数据表明，LJ001 在体外对 TGEV 和 PDCoV 的复制具有抗病毒活性，可作为治疗冠状病毒感染的新候选药物。
