R&D&Innovation Department, EXMceuticals Portugal Lda, Lisboa, Portugal.
Cooperativa de Formação e Animação Cultural - Centre for Interdisciplinary Development and Research on Environment, Applied Management and Space (COFAC-DREAMS)-Universidade Lusófona, Lisboa, Portugal.
Front Immunol. 2022 Feb 15;13:841459. doi: 10.3389/fimmu.2022.841459. eCollection 2022.
In late 2019, COVID-19 emerged in Wuhan, China. Currently, it is an ongoing global health threat stressing the need for therapeutic compounds. Linking the virus life cycle and its interaction with cell receptors and internal cellular machinery is key to developing therapies based on the control of infectivity and inflammation. In this framework, we evaluate the combination of cannabidiol (CBD), as an anti-inflammatory molecule, and terpenes, by their anti-microbiological properties, in reducing SARS-CoV-2 infectivity. Our group settled six formulations combining CBD and terpenes purified from L, , and . The formulations were analyzed by HPLC and GC-MS and evaluated for virucide and antiviral potential by studies in alveolar basal epithelial, colon, kidney, and keratinocyte human cell lines.
We demonstrate the virucide effectiveness of CBD and terpene-based formulations. F2TC reduces the infectivity by 17%, 24%, and 99% for CaCo-2, HaCat, and A549, respectively, and F1TC by 43%, 37%, and 29% for Hek293T, HaCaT, and Caco-2, respectively. To the best of our knowledge, this is the first approach that tackles the combination of CBD with a specific group of terpenes against SARS-CoV-2 in different cell lines. The differential effectiveness of formulations according to the cell line can be relevant to understanding the pattern of virus infectivity and the host inflammation response, and lead to new therapeutic strategies.
在 2019 年末，COVID-19 在中国武汉出现。目前，它是一个持续的全球健康威胁，强调需要治疗化合物。将病毒生命周期与其与细胞受体和细胞内部机制的相互作用联系起来，是开发基于控制传染性和炎症的治疗方法的关键。在这个框架内，我们评估了大麻二酚 (CBD) 和萜烯的组合，通过其抗微生物特性，来降低 SARS-CoV-2 的感染性。我们的小组结合了从 L 、 和 中纯化的 CBD 和萜烯，组合成六种配方。通过 HPLC 和 GC-MS 对配方进行分析，并通过在肺泡基底上皮细胞、结肠、肾脏和角质形成细胞人细胞系中进行的 研究评估其病毒杀灭和抗病毒潜力。
我们证明了 CBD 和萜烯配方的病毒杀灭效果。F2TC 使 CaCo-2、HaCat 和 A549 的感染性分别降低了 17%、24%和 99%，而 F1TC 使 Hek293T、HaCaT 和 Caco-2 的感染性分别降低了 43%、37%和 29%。据我们所知，这是首次针对 SARS-CoV-2 在不同细胞系中使用 CBD 与特定萜烯组合的方法。根据细胞系的配方的不同效果可能与理解病毒感染性模式和宿主炎症反应有关，并为新的治疗策略提供信息。
