Green G M, Jurkowska G, Berube F L, Rivard N, Guan D, Morisset J
Department of Physiology, University of Texas Health Science Center, San Antonio 78284.
Am J Physiol. 1992 Apr;262(4 Pt 1):G740-6. doi: 10.1152/ajpgi.1992.262.4.G740.
The role of cholecystokinin (CCK) in induction and maintenance of pancreatic growth stimulated by a high-protein diet was investigated. Rats adapted to 5% casein diet were switched to 70% casein for 21 days. MK-329, a CCK receptor antagonist, was administered at 2.5 mg.kg-1.day-1 ip, beginning on day zero (day zero treatment) or day 7 (midcourse treatment) of feeding 70% casein and thereafter. Another group was returned to 5% casein after 7 days of feeding 70% casein. Feeding 70% casein significantly stimulated increases of 32, 87, 74, 216, and 1,450% in pancreatic DNA, RNA, wet weight, protein content, and chymotrypsin content, respectively. Midcourse treatment with MK-329 was more effective than day zero treatment, and it completely reversed increases in pancreatic weight and RNA content, partially reversed increases in protein and chymotrypsin content, and had no effect on DNA content. Return to 5% casein rapidly reversed increases in pancreatic parameters, except for DNA. The results indicate that CCK is essential for induction and maintenance of dietary protein-stimulated pancreatic hypertrophy.
研究了胆囊收缩素（CCK）在高蛋白饮食刺激胰腺生长的诱导和维持过程中的作用。将适应5%酪蛋白饮食的大鼠换成70%酪蛋白饮食，持续21天。从喂食70%酪蛋白的第0天（第0天处理）或第7天（中途处理）开始，以2.5 mg.kg-1.day-1的剂量腹腔注射CCK受体拮抗剂MK-329，并在之后持续给药。另一组在喂食70%酪蛋白7天后恢复到5%酪蛋白饮食。喂食70%酪蛋白显著刺激胰腺DNA、RNA、湿重、蛋白质含量和胰凝乳蛋白酶含量分别增加32%、87%、74%、216%和1450%。MK-329中途处理比第0天处理更有效，它完全逆转了胰腺重量和RNA含量的增加，部分逆转了蛋白质和胰凝乳蛋白酶含量的增加，对DNA含量没有影响。恢复到5%酪蛋白饮食迅速逆转了胰腺各项参数的增加，但DNA除外。结果表明，CCK对饮食蛋白刺激的胰腺肥大的诱导和维持至关重要。
