Chernukh A M, Podoprigora G I, Kranchev A K
Biull Eksp Biol Med. 1978 Jun;85(6):654-7.
Peculiarities of permeability of intestinal barrier in germfree animals for enteropathogenic E. coli 055 were studied. Both germfree and conventional guinea pigs and rats were used. An increase of bacteriemia was revealed in gnotobiotes during the first day of oral E. coli 055 monocontamination; bacteriemia was transient in conventional animals. Under electron microscopy alterations of intercellular contacts and formation of spaces between enterocytes containing numerous microorganisms were found in the intestinal mucous membrane in gnotobiotes. Also more pronounced changes of microvessels of the intestinal mucous membrane were discovered in gnotobiotes. The processes of ingestionan and digestion of Escherichia coli by enterocytes and leukocytes were noted in conventional animals. The revealed derangements of the intestinal barrier in gnotobiotes explain the cause of higher bacteriemia in germfree animals. An important role of the microbial factor in the formation of intestinal barrier is indicated by the data obtained.
研究了无菌动物肠道屏障对肠致病性大肠杆菌O55的通透性特点。使用了无菌和常规豚鼠及大鼠。在口服大肠杆菌O55单菌污染的第一天，无菌动物出现菌血症增加；常规动物的菌血症是短暂的。在电子显微镜下，无菌动物的肠黏膜中发现细胞间接触改变以及肠细胞之间形成含有大量微生物的间隙。还发现无菌动物肠黏膜微血管有更明显的变化。在常规动物中观察到肠细胞和白细胞对大肠杆菌的摄取和消化过程。无菌动物肠道屏障的这些紊乱揭示了无菌动物菌血症较高的原因。所获得的数据表明微生物因子在肠道屏障形成中起重要作用。
