Podoprigora G I, Hoffman J, Janacek J, Naprstka J
Biull Eksp Biol Med. 1980 Jul;89(7):17-9.
The level of cAMP in macrophages, intestinal mucosa and blood plasma as well as its formation in intestinal mucosa of germfree animals under the effect of lipopolysaccharide E. coli 055 (LPS) were studied in experiments on germfree and ordinary mice and guinea-pigs. The concentration of cAMP in intestinal mucosa of ordinary guinea-pigs was 5-fold higher than in germfree animals. LPS induced an increase in cAMP level in intestinal mucosa, but this level did not reach that in ordinary animals. The levels of cAMP in blood plasma of germfree guinea-pigs in macrophages of germfree mice increased 2-fold and 4-fold, respectively, 30 minutes following the treatment with LPS. The increased level of cAMP was accompanied by its intensive secretion into the exocellular medium. Macrophages of ordinary animals had a moderate output of cAMP. A conclusion is made about the relationship between cAMP formation and microbial contamination of the microorganism as well as about an important role of the cyclic nucleotide in the mechanism of nonspecific resistance and homeostatic reactions of the body to microbial exposure.
在无菌和普通小鼠及豚鼠身上进行的实验中，研究了脂多糖大肠杆菌055（LPS）作用下无菌动物巨噬细胞、肠黏膜和血浆中cAMP的水平及其在肠黏膜中的形成。普通豚鼠肠黏膜中cAMP的浓度比无菌动物高5倍。LPS诱导肠黏膜中cAMP水平升高，但该水平未达到普通动物的水平。用LPS处理30分钟后，无菌豚鼠血浆中cAMP水平以及无菌小鼠巨噬细胞中cAMP水平分别升高了2倍和4倍。cAMP水平的升高伴随着其向细胞外培养基的大量分泌。普通动物的巨噬细胞cAMP输出量适中。得出了关于cAMP形成与微生物污染之间的关系以及环核苷酸在机体非特异性抵抗和对微生物暴露的稳态反应机制中的重要作用的结论。
