Chen Xianxiang, Xiao Wenhao, Shen Mingyue, Yu Qiang, Chen Yi, Yang Jun, Xie Jianhua
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, China.
Curr Res Food Sci. 2022 Feb 10;5:392-400. doi: 10.1016/j.crfs.2022.01.024. eCollection 2022.
Benth has been consumed as a functional food for many years. It is widely believed that storage times affect its health benefits. In this study, Benth polysaccharides with two different storage times (fresh and storage for 1 year) were prepared, namely, FMP and AMP. The physicochemical properties and bioactivity were comparatively assessed. Results indicated that FMP was mainly composed of galacturonic acid, galactose, and glucose with a molecular weight of 44.39 kDa. AMP was composed of galacturonic acid, galactose, and fructose with a molecular weight of 64.34 kDa. However, the principal structural characteristics of polysaccharides remained stable. Furthermore, assays of antioxidant activity showed that Benth polysaccharide had an antioxidant effect against DPPH radical, ABTS radical cation, among which FMP was stronger. Additionally, flow cytometry indicated that the apoptosis rate of FMP and AMP on HepG2 tumor cells was 22.50 ± 1.25% and 15.49 ± 1.30%, respectively. In general, antioxidant and antitumor activities of Benth polysaccharides were decreased as the storage for 1 year. The change of physicochemical properties was responsible for the enhanced bioactivities. These results explained how polysaccharides contributed to the decreased health benefits of Benth during storage.
多年来，Benth一直被作为功能性食品食用。人们普遍认为储存时间会影响其健康益处。在本研究中，制备了两种不同储存时间（新鲜的和储存1年的）的Benth多糖，即FMP和AMP。对其理化性质和生物活性进行了比较评估。结果表明，FMP主要由半乳糖醛酸、半乳糖和葡萄糖组成，分子量为44.39 kDa。AMP由半乳糖醛酸、半乳糖和果糖组成，分子量为64.34 kDa。然而，多糖的主要结构特征保持稳定。此外，抗氧化活性测定表明，Benth多糖对DPPH自由基、ABTS自由基阳离子具有抗氧化作用，其中FMP的作用更强。此外，流式细胞术表明，FMP和AMP对HepG2肿瘤细胞的凋亡率分别为22.50±1.25%和15.49±1.30%。总体而言，储存1年的Benth多糖的抗氧化和抗肿瘤活性降低。理化性质的变化导致了生物活性的增强。这些结果解释了多糖是如何导致储存期间Benth的健康益处降低的。
