研究小鼠葡萄糖稳态和胰岛功能的方案。
A protocol for studying glucose homeostasis and islet function in mice.
机构信息
Department of Physiology, University of Toronto, 1 King's College Circle, Medical Science Building Rm#3352, Toronto, ON M5S 1A8, Canada.
Division of Advanced Diagnostics, Metabolism, Toronto General Research Institute, Toronto, ON, Canada.
出版信息
STAR Protoc. 2022 Feb 17;3(1):101171. doi: 10.1016/j.xpro.2022.101171. eCollection 2022 Mar 18.
Glucose tolerance test and glucose stimulated insulin secretion are important measures to determine glucose homeostasis and islet function and assess diabetes. Here, we provide a protocol where glucose tolerance test is used to study glucose homeostasis and insulin secretion in vivo, followed by islet isolation and glucose stimulated insulin secretion to determine islet function ex vivo. This protocol enables evaluation of glucose homeostasis and islets in mice which can also be applied to rat, beta cell lines and human studies. For complete details on the use and execution of this profile, please refer to Al Rijjal et al. (2021).
葡萄糖耐量试验和葡萄糖刺激胰岛素分泌是确定血糖稳态和胰岛功能以及评估糖尿病的重要手段。在这里，我们提供了一个方案，使用葡萄糖耐量试验来研究体内的血糖稳态和胰岛素分泌，然后进行胰岛分离和葡萄糖刺激胰岛素分泌来测定体外的胰岛功能。该方案可用于评估小鼠的血糖稳态和胰岛，也可应用于大鼠、β细胞系和人类研究。有关该方案的使用和执行的完整详细信息，请参阅 Al Rijjal 等人（2021 年）。
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