Heikkinen Sami, Argmann Carmen A, Champy Marie-France, Auwerx Johan
A.I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland.
Curr Protoc Mol Biol. 2007 Jan;Chapter 29:Unit 29B.3. doi: 10.1002/0471142727.mb29b03s77.
Obesity and dyslipidemia are often found in association with insulin resistance (IR). These components combined with hypertension characterize the most common endocrine disorder in humans, the metabolic syndrome. Thus, in addition to profiling body weight evolution and lipid metabolites, glucose tolerance (a reflection of IR) and insulin sensitivity should also be considered as part of any metabolic phenotyping protocol. The ability to measure IR and glucose tolerance is important not only in the quest to fully understand the pathogenesis of the metabolic syndrome in the mouse, but also to test the effects of potential interventions. This unit presents a variety of tests used for this purpose, including direct blood glucose measurements, insulin measurement by ELISA, the homeostatic model assessment, glucose tolerance and insulin sensitivity tests, and the euglycemic clamp.
肥胖和血脂异常常与胰岛素抵抗（IR）相关。这些因素与高血压共同构成了人类最常见的内分泌紊乱——代谢综合征的特征。因此，除了分析体重变化和脂质代谢产物外，葡萄糖耐量（IR的一种反映）和胰岛素敏感性也应被视为任何代谢表型分析方案的一部分。测量IR和葡萄糖耐量的能力不仅对于全面理解小鼠代谢综合征的发病机制至关重要，而且对于测试潜在干预措施的效果也很重要。本单元介绍了用于此目的的各种测试，包括直接血糖测量、ELISA法胰岛素测量、稳态模型评估、葡萄糖耐量和胰岛素敏感性测试以及正常血糖钳夹技术。
