组织透明化后进行大容量体积成像：成功所需的必要工具。

Massive volumetric imaging of cleared tissue: The necessary tools to be successful.

机构信息

The Department of Cell Biology and The Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, PA, USA.

出版信息

Int J Biochem Cell Biol. 2019 Jul;112:76-78. doi: 10.1016/j.biocel.2019.05.007. Epub 2019 May 11.

DOI:10.1016/j.biocel.2019.05.007

Abstract

Recent technological innovations in high-speed microscopes, tissue clearing methods, optics and computation have made it possible to image enormous biologic volumes. These techniques are applicable to a broad range of biomedical disciplines and are becoming a necessary tool for exploring biologic systems that extend beyond cellular microenvironments to entire organs and organisms. Here we broadly discuss the tool sets that are available for cleared tissue imaging and how various choices may influence the planning and feasibility of an experiment.

摘要

近年来，高速显微镜、组织透明化方法、光学和计算技术的创新使得对巨大生物体积进行成像成为可能。这些技术适用于广泛的生物医学学科，并且正在成为探索超越细胞微环境的整个器官和生物体的生物系统的必要工具。在这里，我们广泛讨论了用于透明组织成像的工具集，以及各种选择如何影响实验的规划和可行性。