用于鉴定和分析的纯成年小鼠小胶质细胞和星形胶质细胞的分离和培养。
Isolation and culture of pure adult mouse microglia and astrocytes for characterization and analyses.
机构信息
Institute for Molecular Bioscience and IMB Centre for Inflammation and Disease Research, The University of Queensland, St. Lucia, QLD 4072, Australia.
Clem Jones Centre for Ageing and Dementia Research, Queensland Brain Institute, The University of Queensland, St. Lucia, QLD 4072, Australia.
出版信息
STAR Protoc. 2022 Apr 11;3(2):101295. doi: 10.1016/j.xpro.2022.101295. eCollection 2022 Jun 17.
Microglia and astrocytes are implicated in aging and age-related diseases. Here, we present a protocol to isolate and culture these glia cells from the murine brain. The protocol consists of two parts: magnetic sorting of adult microglia and mechanical/magnetic sorting of adult microglia and astrocytes. We then describe the characterization of these glial cells by flow cytometry and immunohistochemistry. Microglia isolated from aged mice maintain age-related phenotype during culture. These purified glia cells can be applied in studies.
小胶质细胞和星形胶质细胞与衰老和与年龄相关的疾病有关。在这里，我们提供了一个从鼠脑中分离和培养这些神经胶质细胞的方案。该方案包括两部分：成年小胶质细胞的磁分选和成年小胶质细胞和星形胶质细胞的机械/磁分选。然后，我们通过流式细胞术和免疫组织化学描述了这些神经胶质细胞的特征。从小鼠脑中分离的小胶质细胞在培养过程中保持与年龄相关的表型。这些纯化的神经胶质细胞可应用于研究中。
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