Suppr超能文献

基于公共基因芯片数据的基因共表达分析。

Gene coexpression analysis in based on public microarray data.

机构信息

Centre of Systems Biology, Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece.

biobank.cy Center of Excellence in Biobanking and Biomedical Research, University of Cyprus, 2029 Nicosia, Cyprus.

出版信息

STAR Protoc. 2022 Feb 26;3(1):101208. doi: 10.1016/j.xpro.2022.101208. eCollection 2022 Mar 18.

Abstract

Coexpressed genes tend to participate in related biological processes. Gene coexpression analysis allows the discovery of functional gene partners or the assignment of biological roles to genes of unknown function. In this protocol, we describe the steps necessary to create a gene coexpression tree for , using publicly available Affymetrix CEL microarray data. Because the computational analysis described here is highly dependent on sample quality, we detail an automatic quality control approach. For complete details on the use and execution of this protocol, please refer to Zogopoulos et al. (2021).

摘要

共表达的基因往往参与相关的生物过程。基因共表达分析允许发现功能基因伙伴,或为未知功能的基因分配生物学角色。在本方案中,我们描述了使用公共可用的 Affymetrix CEL 微阵列数据为 创建基因共表达树所需的步骤。由于这里描述的计算分析高度依赖于样本质量,因此我们详细介绍了一种自动质量控制方法。有关此方案的使用和执行的完整详细信息,请参阅 Zogopoulos 等人。(2021 年)。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98c5/8885756/59c56f4b422a/fx1.jpg

相似文献

1
Gene coexpression analysis in based on public microarray data.
STAR Protoc. 2022 Feb 26;3(1):101208. doi: 10.1016/j.xpro.2022.101208. eCollection 2022 Mar 18.
3
NASCArrays: a repository for microarray data generated by NASC's transcriptomics service.
Nucleic Acids Res. 2004 Jan 1;32(Database issue):D575-7. doi: 10.1093/nar/gkh133.
4
Comparative study of RNA-seq- and microarray-derived coexpression networks in Arabidopsis thaliana.
Bioinformatics. 2013 Mar 15;29(6):717-24. doi: 10.1093/bioinformatics/btt053. Epub 2013 Feb 1.
5
Annotating genes of known and unknown function by large-scale coexpression analysis.
Plant Physiol. 2008 May;147(1):41-57. doi: 10.1104/pp.108.117366. Epub 2008 Mar 19.
6
Coexpression of neighboring genes in the genome of Arabidopsis thaliana.
Genome Res. 2004 Jun;14(6):1060-7. doi: 10.1101/gr.2131104.
7
Transcriptome coexpression analysis using ATTED-II for integrated transcriptomic/metabolomic analysis.
Methods Mol Biol. 2013;1011:317-26. doi: 10.1007/978-1-62703-414-2_25.
8
AGRONOMICS1: a new resource for Arabidopsis transcriptome profiling.
Plant Physiol. 2010 Feb;152(2):487-99. doi: 10.1104/pp.109.150185. Epub 2009 Dec 23.
9
Rank of correlation coefficient as a comparable measure for biological significance of gene coexpression.
DNA Res. 2009 Oct;16(5):249-60. doi: 10.1093/dnares/dsp016. Epub 2009 Sep 18.
10
Transcriptional coordination of the metabolic network in Arabidopsis.
Plant Physiol. 2006 Oct;142(2):762-74. doi: 10.1104/pp.106.080358. Epub 2006 Aug 18.

引用本文的文献

1
ACT2.6: Global Gene Coexpression Network in Using WGCNA.
Genes (Basel). 2025 Feb 23;16(3):258. doi: 10.3390/genes16030258.
2
Phototropin2 3'UTR overlaps with the AT5G58150 gene encoding an inactive RLK kinase.
BMC Plant Biol. 2024 Jan 18;24(1):55. doi: 10.1186/s12870-024-04732-2.
3
HGCA2.0: An RNA-Seq Based Webtool for Gene Coexpression Analysis in .
Cells. 2023 Jan 21;12(3):388. doi: 10.3390/cells12030388.
4
Approaches in Gene Coexpression Analysis in Eukaryotes.
Biology (Basel). 2022 Jul 6;11(7):1019. doi: 10.3390/biology11071019.

本文引用的文献

1
Coexpression Tool: a tool for gene coexpression analysis in .
iScience. 2021 Jul 10;24(8):102848. doi: 10.1016/j.isci.2021.102848. eCollection 2021 Aug 20.
3
MyoMiner: explore gene co-expression in normal and pathological muscle.
BMC Med Genomics. 2020 May 11;13(1):67. doi: 10.1186/s12920-020-0712-3.
4
WebGestalt 2019: gene set analysis toolkit with revamped UIs and APIs.
Nucleic Acids Res. 2019 Jul 2;47(W1):W199-W205. doi: 10.1093/nar/gkz401.
5
InterMineR: an R package for InterMine databases.
Bioinformatics. 2019 Sep 1;35(17):3206-3207. doi: 10.1093/bioinformatics/btz039.
7
Araport: the Arabidopsis information portal.
Nucleic Acids Res. 2015 Jan;43(Database issue):D1003-9. doi: 10.1093/nar/gku1200. Epub 2014 Nov 20.
8
ArrayExpress update--simplifying data submissions.
Nucleic Acids Res. 2015 Jan;43(Database issue):D1113-6. doi: 10.1093/nar/gku1057. Epub 2014 Oct 31.
9
PLANEX: the plant co-expression database.
BMC Plant Biol. 2013 May 20;13:83. doi: 10.1186/1471-2229-13-83.
10
NCBI GEO: archive for functional genomics data sets--update.
Nucleic Acids Res. 2013 Jan;41(Database issue):D991-5. doi: 10.1093/nar/gks1193. Epub 2012 Nov 27.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验