Centre of Systems Biology, Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece.
biobank.cy Center of Excellence in Biobanking and Biomedical Research, University of Cyprus, 2029 Nicosia, Cyprus.
STAR Protoc. 2022 Feb 26;3(1):101208. doi: 10.1016/j.xpro.2022.101208. eCollection 2022 Mar 18.
Coexpressed genes tend to participate in related biological processes. Gene coexpression analysis allows the discovery of functional gene partners or the assignment of biological roles to genes of unknown function. In this protocol, we describe the steps necessary to create a gene coexpression tree for , using publicly available Affymetrix CEL microarray data. Because the computational analysis described here is highly dependent on sample quality, we detail an automatic quality control approach. For complete details on the use and execution of this protocol, please refer to Zogopoulos et al. (2021).
共表达的基因往往参与相关的生物过程。基因共表达分析允许发现功能基因伙伴,或为未知功能的基因分配生物学角色。在本方案中,我们描述了使用公共可用的 Affymetrix CEL 微阵列数据为 创建基因共表达树所需的步骤。由于这里描述的计算分析高度依赖于样本质量,因此我们详细介绍了一种自动质量控制方法。有关此方案的使用和执行的完整详细信息,请参阅 Zogopoulos 等人。(2021 年)。