Department of Biotechnology, Alagappa University, Science Campus, Karaikudi 630 003, Tamil Nadu, India.
Water and Steam Chemistry Division, Bhabha Atomic Research Centre Facilities, Kalpakkam 603 102, Tamil Nadu, India.
Mar Genomics. 2022 Apr;62:100890. doi: 10.1016/j.margen.2021.100890. Epub 2021 Aug 20.
H. boliviensis strain kknpp38 is a dense exopolysaccharide (EPS) producing bacterium, isolated from the early-stage (72-h-old) of marine biofilm. Laboratory experiments demonstrated that this isolate forms a potent biofilm on various artificial substrata viz. polystyrene, stainless steel as well as titanium and possesses high tolerance to chlorine disinfection. To determine the genes and biosynthetic pathways involved in the EPS production, whole-genome sequencing was performed using high-throughput Illumina tag sequencing. The high-quality reads were first de novo assembled using Unicycler genome assembler (version 0.4.9b) and then annotated using Prokka (version 1.13). The complete genome comes from one circular chromosome containing 4.96 Mbp DNA with G + C content of 55%, and encompasses genes encoding 4476 proteins, 2 rRNAs, and 57 tRNAs. Intriguingly, genomic analysis revealed the existence of genes involved in ATP-binding cassette (ABC) transporter-dependent EPS biosynthesis pathways (ugd, ugd2, galU). In addition, we identified genes involved in ectoine (ectA, ectB, ectC, ectD) and polyhydroxyalkanoates (PHAs; fabA, fabB, fabD, fabF, fabH, fabV, fabZ, phaC, phaD, phaG, phaR, phaZ1) production, which are known to involve in bacterial adaptation in saline environment. The outcomes of this study expand scientific understanding on the genes and pathways involved in EPS biosynthesis by marine bacteria.
H. boliviensis 菌株 kknpp38 是一种产生密集胞外多糖（EPS）的细菌，从海洋生物膜的早期（72 小时龄）中分离得到。实验室实验表明，该分离株在各种人工基质（如聚苯乙烯、不锈钢以及钛）上形成强有力的生物膜，并具有高耐氯消毒能力。为了确定参与 EPS 生产的基因和生物合成途径，使用高通量 Illumina 标签测序进行了全基因组测序。使用 Unicycler 基因组组装器（版本 0.4.9b）首次对高质量读数进行从头组装，然后使用 Prokka（版本 1.13）进行注释。完整基因组来自一个包含 4.96 Mbp DNA 的圆形染色体，G+C 含量为 55%，包含编码 4476 种蛋白质、2 种 rRNA 和 57 种 tRNA 的基因。有趣的是，基因组分析显示存在参与 ATP 结合盒（ABC）转运蛋白依赖的 EPS 生物合成途径（ugd、ugd2、galU）的基因。此外，我们还鉴定了参与海藻糖（ectA、ectB、ectC、ectD）和聚羟基烷酸酯（PHAs；fabA、fabB、fabD、fabF、fabH、fabV、fabZ、phaC、phaD、phaG、phaR、phaZ1）生产的基因，这些基因已知涉及细菌在盐环境中的适应。这项研究的结果扩展了对海洋细菌参与 EPS 生物合成的基因和途径的科学认识。
