Telengech Paul, Shahi Sabitree, Kondo Hideki, Suzuki Nobuhiro
Institute of Plant Science and Resources, Okayama University, Kurashiki, 710-0046, Japan.
Arch Virol. 2022 Apr;167(4):1201-1204. doi: 10.1007/s00705-022-05372-3. Epub 2022 Mar 4.
The family Partitiviridae has five genera, among which is the genus Deltapartitivirus. We report here the complete genome sequence of a deltapartitivirus from red clover, termed "red clover cryptic virus 3" (RCCV3). RCCV3 has a bisegmented double-stranded (ds) RNA genome. dsRNA1 and dsRNA2 are 1580 and 1589 nucleotides (nt) in length and are predicted to encode an RNA-directed RNA polymerase (RdRP) and a capsid protein (CP), respectively. The RCCV3 RdRP shares the highest sequence identity with the RdRP of a previously reported deltapartitivirus, Medicago sativa deltapartitivirus 1 (MsDPV1) (76.5%), while the RCCV3 CP shows 50% sequence identity to the CP of MsDPV1. RdRP- and CP-based phylogenetic trees place RCCV3 into a clade of deltapartitiviruses. The sequence and phylogenetic analyses clearly indicate that RCCV3 represents a new species in the genus Deltapartitivirus. RCCV3 was detectable in all three tested cultivars of red clover.
分体病毒科有五个属，其中包括三角洲分体病毒属。我们在此报告一种来自红三叶草的三角洲分体病毒的完整基因组序列，称为“红三叶草隐性病毒3”（RCCV3）。RCCV3具有双分体双链（ds）RNA基因组。dsRNA1和dsRNA2的长度分别为1580和1589个核苷酸（nt），预计分别编码一种RNA指导的RNA聚合酶（RdRP）和一种衣壳蛋白（CP）。RCCV3的RdRP与先前报道的三角洲分体病毒苜蓿三角洲分体病毒1（MsDPV1）的RdRP具有最高的序列同一性（76.5%），而RCCV3的CP与MsDPV1的CP具有50%的序列同一性。基于RdRP和CP的系统发育树将RCCV3归入三角洲分体病毒的一个分支。序列和系统发育分析清楚地表明，RCCV3代表三角洲分体病毒属中的一个新物种。在所有三个测试的红三叶草品种中都可检测到RCCV3。
