An Hongliu, Tan Guanlin, Xiong Guihong, Li Meirong, Fang Shouguo, Islam Saif Ul, Zhang Songbai, Li Fan
Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, 434025, Hubei, China.
Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, 650201, Yunnan, China.
Arch Virol. 2017 Sep;162(9):2897-2901. doi: 10.1007/s00705-017-3421-2. Epub 2017 May 25.
Two double stranded RNAs (dsRNA), likely representing the genome of a novel deltapartitivirus, provisionally named carnation cryptic virus 3 (CCV3), were recovered from Dianthus amurensis. The two dsRNAs were 1,573 (dsRNA1) and 1,561 (dsRNA2) bp in size, each containing a single open reading frame (ORF) encoding a 475- and 411-aa protein, respectively. The 475-aa protein contains a conserved RNA dependent RNA polymerase (RdRp) domain which shows significant homology to RdRps of established or putative partitiviruses, particularly those belonging to the genus Deltapartitivirus. However, it shares an amino acid identity of 75% with its closest relative, the RdRp of the deltapartitivirus beet cryptic virus 2 (BCV2), and is <62% identical to the RdRps of other partitiviruses. In a phylogenetic tree constructed with RdRps of selected partitiviruses, CCV3 clustered with BCV2 and formed a well-supported monophyletic clade with known or putative deltapartitiviruses.
从毛脉石竹中分离出两条双链RNA（dsRNA），可能代表一种新型分体病毒的基因组，暂命名为香石竹隐病毒3（CCV3）。这两条dsRNA的大小分别为1573bp（dsRNA1）和1561bp（dsRNA2），每条都包含一个单一的开放阅读框（ORF），分别编码一个475和411个氨基酸的蛋白质。475个氨基酸的蛋白质包含一个保守的RNA依赖RNA聚合酶（RdRp）结构域，与已确定或推定的分体病毒的RdRp具有显著同源性，特别是那些属于δ分体病毒属的病毒。然而，它与其最密切相关的δ分体病毒甜菜隐病毒2（BCV2）的RdRp的氨基酸同一性为75%，与其他分体病毒的RdRp的同一性小于62%。在用选定分体病毒的RdRp构建的系统发育树中，CCV3与BCV2聚类，并与已知或推定的δ分体病毒形成一个得到充分支持的单系分支。
