Galakatos N G, Daub E, Botstein D, Walsh C T
Biochemistry. 1986 Jun 3;25(11):3255-60. doi: 10.1021/bi00359a026.
The nucleotide sequence of the alr gene encoding the biosynthetic alanine racemase in Salmonella typhimurium is reported. The sequence was determined by the dideoxy chain termination method of Sanger mostly from recombinants derived from shotgun and specific subcloning of a 2.6-kilobase region containing the alr gene. The final bridging of nonoverlapping contiguous sequences was accomplished with the use of synthetic site-specific primers. The alr gene was found to be 1077 base pairs in length encoding a protein of 359 amino acid residues. Comparison of alr with the dadB gene encoding the catabolic alanine racemase in S. typhimurium revealed almost identical size (1077 vs. 1068 base pairs) and 52% sequence identity. The respective gene products displayed 43% homology, which includes a decapeptide bearing the pyridoxal 5'-phosphate binding site.
报道了鼠伤寒沙门氏菌中编码生物合成丙氨酸消旋酶的alr基因的核苷酸序列。该序列主要通过桑格双脱氧链终止法，从包含alr基因的2.6千碱基区域的鸟枪法重组体和特定亚克隆体中确定。非重叠连续序列的最终拼接是使用合成的位点特异性引物完成的。发现alr基因长度为1077个碱基对，编码一个由359个氨基酸残基组成的蛋白质。将alr与鼠伤寒沙门氏菌中编码分解代谢丙氨酸消旋酶的dadB基因进行比较，发现它们大小几乎相同（1077对1068个碱基对），序列同一性为52%。各自的基因产物显示出43%的同源性，其中包括一个带有磷酸吡哆醛结合位点的十肽。
