Krylov O R, Avdeeva Zh I, Podoplelov I I, Medunitsyn N V
Biull Eksp Biol Med. 1986 Jun;101(6):715-7.
The in vitro induction of effector cells mediating delayed type hypersensitivity (DTH) responses was demonstrated during joint cultivation of plastic non-adherent spleen cells of intact CBA mice and adherent spleen cells pretreated with MBSA or OVA. The activity of these effector cells was studied by testing their ability to produce MIF after stimulation by a specific antigen. The formation of MIF-producing cells was observed with the use of low antigen doses (about 50 micrograms/ml). High OVA doses (250 micrograms/ml) were found to suppress the development of MIF-producing cells. MIF-producing cells obtained after either in vitro or in vivo exposure to identical antigens did not differ in their activity.
在完整CBA小鼠的塑料非贴壁脾细胞与经MBSA或OVA预处理的贴壁脾细胞联合培养过程中，证明了介导迟发型超敏反应（DTH）的效应细胞的体外诱导。通过检测这些效应细胞在特定抗原刺激后产生MIF的能力来研究其活性。使用低抗原剂量（约50微克/毫升）时观察到产生MIF的细胞形成。发现高OVA剂量（250微克/毫升）会抑制产生MIF的细胞的发育。在体外或体内暴露于相同抗原后获得的产生MIF的细胞，其活性没有差异。
