Kwon Kiyoon, Jung Junyoung, Sahu Abhishek, Tae Giyoong
School of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea.
School of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea; Department of Biotechnology, National Institute of Pharmaceutical Education & Research (NIPER), Hajipur, 844102, India.
J Control Release. 2022 Apr;344:160-172. doi: 10.1016/j.jconrel.2022.02.033. Epub 2022 Mar 3.
Nanoreactors for scavenging reactive oxygen species (ROS), a major factor in inflammatory diseases, can reduce overproduced ROS, and thus can prevent further progress of the diseases or facilitate the regeneration of damaged inflamed tissues. Herein, we designed a pluronic-based nanocarrier loaded with dual antioxidant enzymes present in vivo (superoxide dismutase (SOD) and catalase (CAT)) as a nanoreactor system for the regeneration of inflammatory tissue. The catalytic activity of each enzyme was enhanced by loading it into the nanocarrier. More importantly, the nanocarrier could enhance the cascade reaction between SOD and CAT, which converts the superoxide anion to oxygen. The synergistic anti-inflammatory effect of the nanoreactor based on the cascade reaction was verified in vitro. Furthermore, in an inflammatory bowel disease (IBD) mouse model, the dual enzyme (SOD/CAT)-loaded nanocarrier could result in significantly enhanced tissue regeneration and notably alleviated inflammation activities upon intravenous administration of them compared to other control groups, including single enzyme (SOD or CAT)-loaded nanocarrier and the free mixture of both enzymes without the nanocarrier. Thus, the efficacy of the nanoreactor for the cascade reaction on tissue regeneration in vivo was proved. Accordingly, the nanoreactor could be applied for tissue regeneration therapy against various inflammatory diseases.
用于清除活性氧（ROS）的纳米反应器是炎症性疾病的主要因素，它可以减少过量产生的ROS，从而可以防止疾病的进一步发展或促进受损炎症组织的再生。在此，我们设计了一种基于普朗尼克的纳米载体，其负载有体内存在的两种抗氧化酶（超氧化物歧化酶（SOD）和过氧化氢酶（CAT）），作为用于炎症组织再生的纳米反应器系统。将每种酶负载到纳米载体中可增强其催化活性。更重要的是，纳米载体可以增强SOD和CAT之间的级联反应，该反应将超氧阴离子转化为氧气。基于级联反应的纳米反应器的协同抗炎作用在体外得到了验证。此外，在炎症性肠病（IBD）小鼠模型中，与其他对照组相比，包括负载单一酶（SOD或CAT）的纳米载体以及不含纳米载体的两种酶的游离混合物，静脉注射负载双酶（SOD/CAT）的纳米载体可显著增强组织再生并显著减轻炎症活动。因此，证明了纳米反应器在体内对组织再生的级联反应的功效。因此，纳米反应器可用于针对各种炎症性疾病的组织再生治疗。
