Asou H, Mutou N, Hirano S, Takagi T, Kajiwara K, Komatsubara J, Sugaya E
Cell Struct Funct. 1986 Jun;11(2):175-80. doi: 10.1247/csf.11.175.
Localization of ganglioside GM1 in cholinergic neurons from the septal area of a primary culture newborn rat brain was studied with a double avidin-biotin complex system. Cholinergic neurons were identified by double immunolabeling techniques that use choline acetyltransferase (ChAT) and neurofilament (NF) protein-antibodies. ChAT-positive neurons also were stained for ganglioside GM1 by using an avidin-biotin complex technique.
利用双抗生物素蛋白-生物素复合物系统研究了新生大鼠原代培养脑隔区胆碱能神经元中神经节苷脂GM1的定位。通过使用胆碱乙酰转移酶（ChAT）和神经丝（NF）蛋白抗体的双重免疫标记技术来鉴定胆碱能神经元。ChAT阳性神经元也通过抗生物素蛋白-生物素复合物技术进行神经节苷脂GM1染色。
