Bioengineering and Environmental Sciences Lab, Department of Energy and Environmental Engineering, CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
Bioengineering and Environmental Sciences Lab, Department of Energy and Environmental Engineering, CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India.
Bioresour Technol. 2022 May;351:126937. doi: 10.1016/j.biortech.2022.126937. Epub 2022 Mar 3.
Addressing the carbon emissions through microbial mediated fermentation is an emerging interest. Custom designed and fabricated gas fermentation (GF) systems were evaluated to optimize the headspace pressure, pH (6.5, 7.5, and 8.5), fermentation time, and substrate concentration by employing enriched homoacetogenic chemolithoautotrophs in non-genetic approach. Headspace pressure showed marked influence on the metabolic conversion of inorganic carbon to acetic and butyric acids with 26% higher productivity than the control (atmospheric pressure). Maximum volatile fatty acid (VFA) yield of 3.7 g/L was observed at alkaline pH (8.5) under 2 bar pressure at carbon load of 10 g/L, 96 h). Acetic (3.0 g/L) and butyric (0.7 g/L) acids were the major products upon conversion of 85% of the inorganic substrate. A better in-situ buffering (β = 0.048) at pH 8.5 along with higher reductive current (RCC: -4.4 mA) depicted better performance of GF towards CO reduction.
通过微生物介导的发酵来解决碳排放问题是一个新兴的关注点。通过采用富集的同型产乙酸化自养菌，采用非遗传方法设计和制造了定制的气体发酵 (GF) 系统，以优化顶空压力、pH 值（6.5、7.5 和 8.5）、发酵时间和底物浓度。顶空压力对无机碳向乙酸和丁酸的代谢转化有显著影响，比对照（大气压）提高了 26%的生产力。在碳负荷为 10 g/L、96 h 时，在碱性 pH 值（8.5）和 2 bar 压力下，最大挥发性脂肪酸 (VFA) 产量为 3.7 g/L。在 85%的无机底物转化过程中，主要产物为乙酸（3.0 g/L）和丁酸（0.7 g/L）。在 pH 值为 8.5 时，更好的原位缓冲（β=0.048）和更高的还原电流（RCC：-4.4 mA）表明 GF 对 CO 还原的性能更好。
