Ishida K, Hinshaw L B
Circ Shock. 1986;19(3):301-8.
We have determined the effect of lethal E. coli infusions in dogs on plasma concentrations of pancreatic and gastrointestinal-derived glucagon and have explored the contributions of each source of glucagon during the early and recovery phases of shock. We examined 18 adult dogs in three protocols: group I received LD100 E. coli alone, group II received LD100 E. coli + tobramycin (TOB), and group III received LD100 E. coli + TOB + methylprednisolone sodium succinate (MPSS). E. coli organisms were infused intravenously during a 1-hour period and each animal was monitored for 6 hours and observed for a 7-day recovery period. Plasma concentrations of pancreatic and gastrointestinal glucagon were determined by specific RIAs. The survival percentages (greater than 7 days) were 0% in group I, 17% in group II, and 83% in group III. Early progressive increases in plasma concentrations of pancreatic and gastrointestinal-derived glucagon, reaching statistical significance by 6 hours following the onset of E. coli administration, were seen in the three groups. The increase in gastrointestinal-derived glucagon was of a greater magnitude than that from the pancreas. Attenuation of the increase appeared to be achieved by corticosteroid infusion during its time of administration (6 hours). Recovery from shock was characterized by an exceptionally slow return (greater than or equal to 7 days) to control levels of glucagon in all recovering animals.
我们已确定致死剂量的大肠杆菌输注对犬血浆中胰腺和胃肠道来源胰高血糖素浓度的影响，并探讨了休克早期和恢复阶段每种胰高血糖素来源的作用。我们采用三种方案对18只成年犬进行了研究：第一组仅接受100%致死剂量的大肠杆菌，第二组接受100%致死剂量的大肠杆菌+妥布霉素（TOB），第三组接受100%致死剂量的大肠杆菌+TOB+琥珀酸钠甲泼尼龙（MPSS）。在1小时内静脉输注大肠杆菌，每只动物监测6小时，并观察7天的恢复期。通过特异性放射免疫分析法测定血浆中胰腺和胃肠道胰高血糖素的浓度。第一组的存活率（超过7天）为0%，第二组为17%，第三组为83%。三组均出现血浆中胰腺和胃肠道来源的胰高血糖素浓度早期逐渐升高，在给予大肠杆菌后6小时达到统计学显著水平。胃肠道来源的胰高血糖素的升高幅度大于胰腺来源的。在给予皮质类固醇（6小时）期间，似乎实现了升高幅度的减弱。所有恢复动物休克恢复的特征是胰高血糖素水平异常缓慢地恢复（大于或等于7天）至对照水平。
