Department of Animal Molecular Biology, National Research Institute of Animal Production, ul Krakowska 1, 32-083, Balice, Poland.
Department of Animal Nutrition and Feed Science, National Research Institute of Animal Production, ul Krakowska 1, 32-083, Balice, Poland.
Mol Biol Rep. 2022 Jul;49(7):6919-6929. doi: 10.1007/s11033-022-07303-6. Epub 2022 Mar 5.
Circulating miRNA molecules are intensively studied for their usefulness as biomarkers of civilization diseases. Meanwhile, it is known that diet can influence the level of miRNA expression in tissues.
Our research aimed to determine how a diet containing various sources of fat (rapeseed oil, beef tallow, coconut oil) and different amounts of cDDGS (corn Dried Distilled Grains with Solubles) affects the miRNA profile in pig fat-the main source of circulating miRNAs. For this purpose, we used Next Generation Sequencing of miRNA libraries.
We observed the highest number of differentially expressed miRNAs in the samples from animals that were fed with coconut oil in the diet compared to all other treatments. In contrary, cDDGS appeared to have little effect on miRNA expression.
We propose a subset of diet-related, adipose-specific, conservative miRNAs among mammals, namely: ssc-miR-99b, ssc-miR-4334-3p, ssc-miR-146b, ssc-miR-23a. Moreover, we observed that several miRNAs regulated by dietary fats are considered as biomarkers in human and animal diseases.
循环 miRNA 分子作为文明病生物标志物的有用性受到了广泛研究。同时，已知饮食可以影响组织中 miRNA 表达水平。
我们的研究旨在确定含有不同来源脂肪（菜籽油、牛脂、椰子油）和不同量 cDDGS（玉米干酒糟及其可溶物）的饮食如何影响猪脂肪中的 miRNA 谱-循环 miRNA 的主要来源。为此，我们使用了 miRNA 文库的下一代测序。
与所有其他处理相比，我们在饮食中用椰子油喂养的动物的样本中观察到最多数量的差异表达 miRNA。相反，cDDGS 似乎对 miRNA 表达影响很小。
我们提出了哺乳动物中一组与饮食相关的、脂肪特异性的保守 miRNA，即：ssc-miR-99b、ssc-miR-4334-3p、ssc-miR-146b、ssc-miR-23a。此外，我们观察到几种受膳食脂肪调节的 miRNA 被认为是人类和动物疾病的生物标志物。
