Biomedical Engineering Laboratory, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, 50670-000, Recife, Brazil.
Biomedical Engineering Laboratory, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, 50670-000, Recife, Brazil.
Colloids Surf B Biointerfaces. 2018 Dec 1;172:272-279. doi: 10.1016/j.colsurfb.2018.08.050. Epub 2018 Aug 24.
An electrochemical immunosensor devoted the core hepatitis B antibody detection, based on polytyramine (PTy) and carbon nanotubes (CNT) composite was developed. The antibody interactions with immobilized antigens were detected by reduction on the electron transfer from ionic species coming from reactive amine groups of PTy. The synthesis in acid medium of PTy-CNT composite favorite a great amount of NH ionic species, forming a nanocomposite with high catalytic activity on the electrode surface. As proof-of-concept, antibodies against the core hepatitis B virus were label-free and reagentless electrochemically detected by square wave voltammetry (SWV) through decrease on cathodic peaks. It was recently reported that hepatitis B core antigen antibodies (anti-HBc) is a powerful biomarker for hepatitis B virus (HBV) infection, being more specific than HBsAg due to the possibility of detecting the occult HBV infections. The nanostructured film was characterized by atomic force microscopy and electrochemical techniques. This immunosensor showed linear responses from 1.0 to 5.0 ng mL and a limit of detection of 0.89 ng mL anti-HBc. It was also tested in assays with negative and positive blood samples using 0.1 M KCl as electrolyte support on readings showing specific responses. This easy reagentless detection platform, showing a remarkable potential to development of bolder and simpler HBV assay for screening of blood bags, in attempting to circumvent point-of-care testing limitations.
基于聚邻苯二胺（PTy）和碳纳米管（CNT）复合材料开发了一种用于核心乙型肝炎抗体检测的电化学免疫传感器。通过来自 PTy 的反应性胺基团的离子物种的电子转移还原来检测抗体与固定化抗原的相互作用。在酸性介质中合成的 PTy-CNT 复合材料有利于大量 NH 离子物种，在电极表面形成具有高催化活性的纳米复合材料。作为概念验证，通过方波伏安法（SWV）通过减少阴极峰来无标记和无试剂地检测针对乙型肝炎病毒核心的抗体（抗-HBc）。最近有报道称，乙型肝炎核心抗原抗体（抗-HBc）是乙型肝炎病毒（HBV）感染的有力生物标志物，由于能够检测隐匿性 HBV 感染，因此比 HBsAg 更具特异性。使用原子力显微镜和电化学技术对纳米结构薄膜进行了表征。该免疫传感器在 1.0 至 5.0 ng mL 的线性响应范围内，检测限为 0.89 ng mL 抗-HBc。还使用阴性和阳性血液样本在含有 0.1 M KCl 的电解质支持下进行了测定，读数显示出特异性反应。这种无需试剂的简便检测平台显示出用于筛选血袋的更简单、更简单的 HBV 检测方法的巨大潜力，旨在克服即时检测的局限性。
