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RNA干扰分析揭示了铁蛋白在日本沼虾睾丸发育中的正向调控作用。

RNA Interference Analysis Reveals the Positive Regulatory Role of Ferritin in Testis Development in the Oriental River Prawn, .

作者信息

Jin Shubo, Fu Hongtuo, Jiang Sufei, Xiong Yiwei, Qiao Hui, Zhang Wenyi, Gong Yongsheng, Wu Yan

机构信息

Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.

出版信息

Front Physiol. 2022 Feb 18;13:805861. doi: 10.3389/fphys.2022.805861. eCollection 2022.

DOI:10.3389/fphys.2022.805861
PMID:35250613
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8896479/
Abstract

Ferritin plays an essential role in organismic and cellular iron homeostasis in . In this study, we aimed to investigate the role of ferritin in the sexual development of male . . According to the qPCR analysis of different tissues and developmental stages, ferritin exhibited high expression levels in the testis and androgenic gland, from post-larval developmental stage 5 (PL5) to PL15, indicating that it may be involved in gonad differentiation and development, especially in male sexual development. hybridization and qPCR analysis in various reproductive cycles of the testis indicated that ferritin may play an essential role in spermatogonia development in . . RNAi analysis revealed that ferritin positively affected mRNA expression of the insulin-like androgenic gland () and the secretion of testosterone, and thus positively affected testis development in . This study highlighted the functions of ferritin in the sexual development of male and provided important information for the establishment of a technique to regulate the process of testis development in .

摘要

铁蛋白在生物和细胞的铁稳态中起着至关重要的作用。在本研究中，我们旨在探究铁蛋白在雄性[物种名称]性发育中的作用。根据对不同组织和发育阶段的qPCR分析，从幼虫发育后期第5阶段（PL5）到PL15，铁蛋白在睾丸和雄性腺中呈现高表达水平，表明它可能参与性腺分化和发育，尤其是雄性性发育。睾丸不同生殖周期的[原位]杂交和qPCR分析表明，铁蛋白可能在[物种名称]的精原细胞发育中起关键作用。RNA干扰分析显示，铁蛋白对胰岛素样雄性腺（[英文缩写]）的mRNA表达和睾酮分泌有正向影响，从而对[物种名称]的睾丸发育产生正向影响。本研究突出了铁蛋白在雄性[物种名称]性发育中的功能，并为建立调控[物种名称]睾丸发育过程的技术提供了重要信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f40f/8896479/fa8390cdfdd1/fphys-13-805861-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f40f/8896479/dffe1ed743e9/fphys-13-805861-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f40f/8896479/d75158b70786/fphys-13-805861-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f40f/8896479/07b38db8681a/fphys-13-805861-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f40f/8896479/fa8390cdfdd1/fphys-13-805861-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f40f/8896479/dffe1ed743e9/fphys-13-805861-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f40f/8896479/d75158b70786/fphys-13-805861-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f40f/8896479/07b38db8681a/fphys-13-805861-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f40f/8896479/fa8390cdfdd1/fphys-13-805861-g004.jpg

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