Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
Genes (Basel). 2022 Nov 9;13(11):2079. doi: 10.3390/genes13112079.
() plays essential roles in cell proliferation and promotes gonad development in many crustaceans. The goal of this study was to investigate the regulatory roles of this gene in the reproductive development of male oriental river prawns (). A phylo-genetic tree analysis revealed that the protein sequence of - was most closely related to those of freshwater prawns, whereas the evolutionary distance from crabs was much longer. A quantitative PCR analysis showed that the expression of - was highest in the gonad of both male and female prawns compared to that in other tissues ( < 0.05), indicating that this gene may play essential roles in the regulation of both testis and ovary development in . In males, - expression in the testis and androgenic gland was higher during the reproductive season than during the non-reproductive season ( < 0.05), implying that plays essential roles in the reproductive development of male . An RNA interference analysis revealed that the expression decreased as the expression decreased, and that few sperm were detected 14 days after the treatment, indicating that positively affects testis development in . The results of this study highlight the functions of in . , and they can be applied to studies of male reproductive development in other crustacean species.
（）在细胞增殖中发挥重要作用，并促进许多甲壳动物的性腺发育。本研究旨在探讨该基因在雄性东方沼虾（）生殖发育中的调控作用。系统发育树分析显示，-的蛋白序列与淡水虾的蛋白序列最为相似，而与蟹的进化距离则要长得多。定量 PCR 分析表明，-在雄性和雌性虾的性腺中的表达均显著高于其他组织（<0.05），表明该基因可能在调节虾的睾丸和卵巢发育中发挥重要作用。在雄性虾中，-在生殖季节的睾丸和精巢腺中的表达高于非生殖季节（<0.05），这表明在雄性虾的生殖发育中发挥重要作用。RNA 干扰分析表明，随着-的表达下降，-的表达也下降，并且在处理 14 天后几乎检测不到精子，表明-对虾的睾丸发育有积极影响。本研究结果突出了在虾中的功能，它们可应用于其他甲壳动物雄性生殖发育的研究。
