• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

保幼激素环氧水解酶基因2在雌性克氏原螯虾生殖过程中的调控作用

Regulation Roles of Juvenile Hormone Epoxide Hydrolase Gene 2 in the Female River Prawn Reproductive Process.

作者信息

Wang Jisheng, Zhang Mengying, Fu Hongtuo, Zhang Wenyi, Xiong Yiwei, Jin Shubo, Qiao Hui, Jiang Sufei

机构信息

Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.

Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.

出版信息

Curr Issues Mol Biol. 2024 Nov 25;46(12):13456-13470. doi: 10.3390/cimb46120803.

DOI:10.3390/cimb46120803
PMID:39727931
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11726744/
Abstract

In this study, we investigated the regulatory roles of the () gene in the reproductive process of female . Its total cDNA length was 1848 bp, encoding for 460 amino acids. It contained conserved domains typical of epoxide hydrolases, such as the Abhydrolase family domain, the EHN epoxide hydrolase superfamily domain, and the "WWG" and "HGWP" motifs. The qPCR results showed that the expression of was the highest in hepatopancreas. was expressed at all stages of the embryonic and larval stages. The expression of at different developmental periods of the ovary was positively correlated with ovarian maturation. In situ hybridization showed that it was mainly located in the cytoplasmic membrane and nucleus of oocytes. The RNA interference technique was used to study the role of in the process of ovarian maturation. The knockdown of with dsRNA in the experimental group resulted in a significant decrease in the percentage of ovaries exceeding stage O-III and the gonadal index compared with the control group. On day 14 (the second molt), the molt frequency was significantly higher in the control group than in the experimental group. The results showed that played an important role in ovarian maturation and molting.

摘要

在本研究中，我们调查了（）基因在雌性（物种名称未给出）生殖过程中的调控作用。其cDNA全长1848 bp，编码460个氨基酸。它包含环氧化物水解酶典型的保守结构域，如Abhydrolase家族结构域、EHN环氧化物水解酶超家族结构域以及“WWG”和“HGWP”基序。qPCR结果表明，（基因名称未给出）在肝胰腺中的表达最高。（基因名称未给出）在胚胎和幼虫阶段的所有时期均有表达。卵巢不同发育时期（基因名称未给出）的表达与卵巢成熟呈正相关。原位杂交显示它主要位于卵母细胞的细胞质膜和细胞核中。采用RNA干扰技术研究（基因名称未给出）在卵巢成熟过程中的作用。与对照组相比，实验组用dsRNA敲低（基因名称未给出）后，超过O - III期的卵巢百分比和性腺指数显著降低。在第14天（第二次蜕皮），对照组的蜕皮频率显著高于实验组。结果表明，（基因名称未给出）在卵巢成熟和蜕皮中起重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5db9/11726744/23d0f3105286/cimb-46-00803-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5db9/11726744/31f76a5e480b/cimb-46-00803-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5db9/11726744/036a59a94958/cimb-46-00803-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5db9/11726744/d4f88e804711/cimb-46-00803-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5db9/11726744/83de5ac4f126/cimb-46-00803-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5db9/11726744/4619a0b6f7d4/cimb-46-00803-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5db9/11726744/5f2dc57544b9/cimb-46-00803-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5db9/11726744/f782671601c8/cimb-46-00803-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5db9/11726744/23d0f3105286/cimb-46-00803-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5db9/11726744/31f76a5e480b/cimb-46-00803-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5db9/11726744/036a59a94958/cimb-46-00803-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5db9/11726744/d4f88e804711/cimb-46-00803-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5db9/11726744/83de5ac4f126/cimb-46-00803-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5db9/11726744/4619a0b6f7d4/cimb-46-00803-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5db9/11726744/5f2dc57544b9/cimb-46-00803-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5db9/11726744/f782671601c8/cimb-46-00803-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5db9/11726744/23d0f3105286/cimb-46-00803-g008.jpg

相似文献

1
Regulation Roles of Juvenile Hormone Epoxide Hydrolase Gene 2 in the Female River Prawn Reproductive Process.保幼激素环氧水解酶基因2在雌性克氏原螯虾生殖过程中的调控作用
Curr Issues Mol Biol. 2024 Nov 25;46(12):13456-13470. doi: 10.3390/cimb46120803.
2
NPC Intracellular Cholesterol Transporter 1 Regulates Ovarian Maturation and Molting in Female .NPC 细胞内胆固醇转运蛋白 1 调控雌性. 的卵巢成熟和蜕皮
Int J Mol Sci. 2024 May 31;25(11):6049. doi: 10.3390/ijms25116049.
3
RNA interference shows that Spook, the precursor gene of 20-hydroxyecdysone (20E), regulates the molting of Macrobrachium nipponense.RNA 干扰表明，Spook（20-羟基蜕皮酮（20E）的前体基因）调节日本沼虾的蜕皮。
J Steroid Biochem Mol Biol. 2021 Oct;213:105976. doi: 10.1016/j.jsbmb.2021.105976. Epub 2021 Aug 18.
4
Functional Study of the Role of the Gene in the Ovarian Development of .基因在 卵巢发育中的功能研究。
Int J Mol Sci. 2024 Jul 3;25(13):7318. doi: 10.3390/ijms25137318.
5
Plays a Vital Role in of Ovary Maturation: Identification, Characterization, and Function Analysis.在卵巢成熟中发挥重要作用：鉴定、特性描述和功能分析。
Genes (Basel). 2022 Aug 21;13(8):1495. doi: 10.3390/genes13081495.
6
Molecular characterization and developmental expression of vitellogenin in the oriental river prawn Macrobrachium nipponense and the effects of RNA interference and eyestalk ablation on ovarian maturation.日本沼虾卵黄蛋白原的分子特征、发育表达及RNA干扰和眼柄切除对卵巢成熟的影响
Gene. 2015 May 10;562(1):22-31. doi: 10.1016/j.gene.2014.12.008. Epub 2014 Dec 8.
7
Function analysis and molecular characterization of cyclin A in ovary development of oriental river prawn, Macrobrachium nipponense.细胞周期蛋白 A 在日本沼虾卵巢发育中的功能分析和分子特征。
Gene. 2021 Jul 1;788:145583. doi: 10.1016/j.gene.2021.145583. Epub 2021 Mar 20.
8
A Novel Legumain-Like Protease in : Identification, Characterization, and Function Analysis in Ovary Maturation.在 中一种新型的组织蛋白酶样蛋白酶的鉴定、特性分析及其在卵巢成熟过程中的功能分析。
Front Endocrinol (Lausanne). 2022 Mar 24;13:858726. doi: 10.3389/fendo.2022.858726. eCollection 2022.
9
Identification and characterization of opsin gene and its role in ovarian maturation in the oriental river prawn Macrobrachium nipponense.日本沼虾视蛋白基因的鉴定、特征分析及其在卵巢成熟中的作用
Comp Biochem Physiol B Biochem Mol Biol. 2018 Apr;218:1-12. doi: 10.1016/j.cbpb.2017.12.016. Epub 2018 Jan 5.
10
Molecular Characterization of a Novel in and Its Function in Ovary Maturation.新型 基因的分子特征及其在卵巢成熟中的功能。
Front Endocrinol (Lausanne). 2022 Jan 10;12:816813. doi: 10.3389/fendo.2021.816813. eCollection 2021.

本文引用的文献

1
Juvenile hormone suppresses the FoxO-takeout axis to shorten longevity in male silkworm.保幼激素通过抑制FoxO-外卖轴缩短雄蚕寿命。
Pestic Biochem Physiol. 2023 May;192:105388. doi: 10.1016/j.pestbp.2023.105388. Epub 2023 Mar 11.
2
Toxicities of polystyrene microplastics (MPs) and hexabromocyclododecane (HBCD), alone or in combination, to the hepatopancreas of the whiteleg shrimp, Litopenaeus vannamei.聚苯乙烯微塑料（MPs）和六溴环十二烷（HBCD）单独或联合对凡纳滨对虾肝胰腺的毒性。
Environ Pollut. 2023 Jul 15;329:121646. doi: 10.1016/j.envpol.2023.121646. Epub 2023 Apr 25.
3
Cloning and characterization of Aedes aegypti juvenile hormone epoxide hydrolases (JHEHs).
埃及伊蚊保幼激素环氧水解酶（JHEHs）的克隆与特性分析
Arch Insect Biochem Physiol. 2023 Jan;112(1):e21977. doi: 10.1002/arch.21977. Epub 2022 Oct 18.
4
Temperature and metal ions regulate larval diapause termination via the 20-hydroxyecdysone and juvenile hormone pathways in Monochamus alternatus.温度和金属离子通过松墨天牛的20-羟基蜕皮激素和保幼激素途径调节幼虫滞育的终止。
Pest Manag Sci. 2023 Jan;79(1):437-446. doi: 10.1002/ps.7212. Epub 2022 Oct 19.
5
Hepatopancreas transcriptome analyses provide new insights into the molecular regulatory mechanism of fast ovary maturation in Macrobrachium nipponense.对肝胰腺转录组的分析为日本沼虾快速卵巢成熟的分子调控机制提供了新的见解。
BMC Genomics. 2022 Aug 31;23(1):625. doi: 10.1186/s12864-022-08851-8.
6
Cloning and Characterization of Juvenile Hormone Epoxide Hydrolases (JHEH) and Their Promoters.蜕皮激素环氧化物水解酶（JHEH）及其启动子的克隆与鉴定。
Biomolecules. 2022 Jul 16;12(7):991. doi: 10.3390/biom12070991.
7
The juvenile hormone epoxide hydrolase homolog in Penaeus vannamei plays immune-related functions.凡纳滨对虾中的保幼激素环氧水解酶同源物发挥免疫相关功能。
Dev Comp Immunol. 2022 Jul;132:104410. doi: 10.1016/j.dci.2022.104410. Epub 2022 Apr 6.
8
RNA Interference Analysis Reveals the Positive Regulatory Role of Ferritin in Testis Development in the Oriental River Prawn, .RNA干扰分析揭示了铁蛋白在日本沼虾睾丸发育中的正向调控作用。
Front Physiol. 2022 Feb 18;13:805861. doi: 10.3389/fphys.2022.805861. eCollection 2022.
9
The vitellogenin receptor functionality of the migratory locust depends on its phosphorylation by juvenile hormone.迁移性蝗虫的卵黄蛋白原受体功能取决于其被保幼激素磷酸化。
Proc Natl Acad Sci U S A. 2021 Sep 14;118(37). doi: 10.1073/pnas.2106908118.
10
Effects of cadmium on antioxidant and non-specific immunity of Macrobrachium nipponense.镉对日本沼虾抗氧化及非特异性免疫的影响。
Ecotoxicol Environ Saf. 2021 Aug 19;224:112651. doi: 10.1016/j.ecoenv.2021.112651.