Grand R J, Gallimore P H
EMBO J. 1986 Jun;5(6):1253-60. doi: 10.1002/j.1460-2075.1986.tb04354.x.
The level of expression of thymidine kinase (TK), heat shock protein 70 (HSP70), beta-tubulin and p53 was assessed in human embryo kidney cells (HEKs) infected with adenovirus type 12 (Ad 12) and Ad 12 early region 1 (E1) mutants. HSP70, beta-tubulin and p53 levels were unchanged but TK activity was dramatically increased following wild-type infection. The initial activation of TK required the expression of the product of the E1A 13S mRNA but sustained expression only occurred with those viruses expressing the E1B proteins as well. A number of human cell lines transformed with either Ad 12 or Ad 5 E1 DNA were also assessed for the level of expression of HSP70, beta-tubulin and p53. Both HSP70 and beta-tubulin levels were greatly increased compared with primary human cells although there was considerable variation between lines. p53 was only expressed at high levels in Ad 12-transformed lines expressing E1A and E1B proteins.
在感染12型腺病毒（Ad 12）和Ad 12早期区域1（E1）突变体的人胚肾细胞（HEK）中，评估了胸苷激酶（TK）、热休克蛋白70（HSP70）、β-微管蛋白和p53的表达水平。野生型感染后，HSP70、β-微管蛋白和p53水平未发生变化，但TK活性显著增加。TK的初始激活需要E1A 13S mRNA产物的表达，但只有那些同时表达E1B蛋白的病毒才能持续表达。还评估了一些用Ad 12或Ad 5 E1 DNA转化的人细胞系中HSP70、β-微管蛋白和p53的表达水平。与原代人细胞相比，HSP70和β-微管蛋白水平均显著升高，尽管各细胞系之间存在相当大的差异。p53仅在表达E1A和E1B蛋白的Ad 12转化细胞系中高水平表达。
