State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou 510006, China.
ZJU-Hangzhou Global Scientific and Technological Innovation Center, Department of Chemistry, Zhejiang University, Hangzhou 310058, China.
Nano Lett. 2022 Mar 23;22(6):2479-2489. doi: 10.1021/acs.nanolett.2c00258. Epub 2022 Mar 7.
New strategies for intracellular electrophysiology break the spatiotemporal limitation of the action potential and lead a notable advance in the investigation of electrically excitable cells and their network. Although successful applications of intracellular recording have been achieved by 3D micro/nanodevices, complex micro/nanofabrication processes preclude the progress of extensive applications. We address this challenge by introducing porous polyethylene terephthalate (PET) membrane to develop a new type of nanotemplate electrode. This nanotemplate electrode is manufactured following a fabrication process on a porous PET membrane by atomic layer deposition. The 3D nanotemplate electrodes afford intracellular access to cardiomyocytes to report intracellular-like action potentials. These controllable nanotemplate electrodes exhibit sensitive and prolonged intracellular recordings of action potentials compared with free-growing 3D nanoelectrodes. This study indicates that the optimized structure of the nanoelectrode significantly promotes the performance of intracellular recording to assess electrophysiology in the fields of cardiology and neuroscience at an action potential level.
新的细胞内电生理学策略打破了动作电位的时空限制，为电兴奋细胞及其网络的研究带来了显著的进步。尽管 3D 微/纳器件已经成功地实现了细胞内记录的应用，但复杂的微/纳制造工艺阻碍了广泛应用的进展。我们通过引入多孔聚对苯二甲酸乙二醇酯（PET）膜来开发新型纳米模板电极来解决这一挑战。这种纳米模板电极是通过在多孔 PET 膜上进行原子层沉积来制造的。3D 纳米模板电极允许心肌细胞进行细胞内访问，以报告类似细胞内的动作电位。与自由生长的 3D 纳米电极相比，这些可控纳米模板电极对动作电位的敏感和持久的细胞内记录。本研究表明，纳米电极的优化结构显著提高了细胞内记录的性能，从而在心脏病学和神经科学领域以动作电位水平评估电生理学。
