State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Science, Guangxi Normal University, Guilin, 541004, PR China; College of Food and Bioengineering, Hezhou University, Hezhou, 542899, PR China.
College of Food and Bioengineering, Hezhou University, Hezhou, 542899, PR China.
Anal Chim Acta. 2022 Apr 1;1200:339604. doi: 10.1016/j.aca.2022.339604. Epub 2022 Feb 14.
Nanozyme-based colorimetric sensing has attracted significant interest in recent years, and a number of redox-type nanozyme-based colorimetric sensors based on peroxidase and oxidase mimics have been reported. However, conventional redox-type nanozyme-based colorimetric sensing is affected by interference from the endogenous reductants present in actual samples. Herein, we describe the development of a homogeneous nonredox-type nanozyme-based colorimetric sensor that exploits the intrinsic phosphatase-like activity of CeO. Specifically, colorimetric detection platforms for fluoride ions, zearalenone, and hydrogen peroxide were constructed based on activity inhibition, aptamer-assisted gate control, and plasmonic nanoparticle growth, respectively. Thus, this study reports a versatile route for constructing nonredox nanozyme-based colorimetric sensors that are not affected by interference from endogenous reductants and are thus highly promising for use in food safety and bioassay applications.
基于纳米酶的比色传感近年来受到了广泛关注，已有许多基于过氧化物酶和氧化酶模拟物的氧化还原型纳米酶比色传感器被报道。然而，传统的氧化还原型纳米酶比色传感受到实际样品中内源性还原剂的干扰。在此，我们描述了一种均相非氧化还原型纳米酶比色传感器的开发，该传感器利用了 CeO 的内在磷酸酶样活性。具体而言，分别基于活性抑制、适体辅助门控和等离子体纳米颗粒生长构建了用于氟离子、玉米赤霉烯酮和过氧化氢的比色检测平台。因此，本研究报告了一种构建不受内源性还原剂干扰的非氧化还原纳米酶比色传感器的通用途径，因此在食品安全和生物分析应用中具有很高的应用前景。
