Jiangsu Key Laboratory of New Power Batteries, National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, P. R. China.
Anhui Engineering Technology Research Center of Biochemical Pharmaceutical, School of Pharmacy, Bengbu Medical University, Bengbu, 233030, P. R. China.
Anal Bioanal Chem. 2024 Nov;416(27):5985-5992. doi: 10.1007/s00216-024-05462-y. Epub 2024 Aug 1.
Hydrolytic nanozyme-based visual colorimetry has emerged as a promising strategy for the detection of aluminum ions. However, most studies focus on simulating the structure of natural enzymes while neglecting to regulate the rate of hydrolysis-related steps, leading to low enzyme-like activity for hydrolytic nanozymes. Herein, we constructed a ruthenium dioxide (RuO) in situ embedded cerium oxide (CeO) nanozyme (RuO/CeO) with a Lewis acid-base pair (Ce-O-Ru-OH), which can simulate the catalytic behavior of phosphatase (PPase) and can be quantitatively quenched by Al to achieve accurate and sensitive Al colorimetric sensing detection. The incorporation of Ru into CeO nanorods accelerates the dissociation of HO, followed by subsequent combination of hydroxide species to Lewis acidic Ce-O sites. This synergistic effect facilitates substrate activation and significantly enhances the hydrolysis activity of the nanozyme. The results show that the RuO/CeO nanozyme exhibits a limit of detection as low as 0.5 ng/mL. We also demonstrate their efficacy in detecting Al in various practical food samples. This study offers novel insights into the advancement of highly sensitive hydrolytic nanozyme engineering for sensing applications.
基于水解纳米酶的比色法已成为一种很有前途的用于检测铝离子的策略。然而，大多数研究都集中在模拟天然酶的结构，而忽略了调节与水解相关步骤的速率，导致水解纳米酶的类酶活性较低。在此，我们构建了一种二氧化钌（RuO）原位嵌入氧化铈（CeO）纳米酶（RuO/CeO），其中含有路易斯酸碱对（Ce-O-Ru-OH），可以模拟磷酸酶（PPase）的催化行为，并可被 Al 定量猝灭，从而实现对 Al 的精确和灵敏的比色传感检测。Ru 掺入 CeO 纳米棒中会加速 HO 的解离，随后氢氧化物物种与路易斯酸性的 Ce-O 位点结合。这种协同作用促进了底物的活化，显著提高了纳米酶的水解活性。结果表明，RuO/CeO 纳米酶的检测限低至 0.5ng/mL。我们还证明了它们在各种实际食品样品中检测 Al 的功效。该研究为用于传感应用的高灵敏度水解纳米酶工程的发展提供了新的见解。
