淋巴细胞性脉络丛脑膜炎病毒WE株糖蛋白的重组表达：一个氨基酸决定差异。
Recombinant expression of lymphocytic choriomeningitis virus strain WE glycoproteins: a single amino acid makes the difference.
作者信息
Beyer W R, Miletic H, Ostertag W, von Laer D
机构信息
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, D-20251 Hamburg, Germany.
出版信息
J Virol. 2001 Jan;75(2):1061-4. doi: 10.1128/JVI.75.2.1061-1064.2001.
Abstract
Cytoplasmic vector systems are generally used for expression of lymphocytic choriomeningitis virus (LCMV) proteins. However, we achieved high levels of cell surface glycoproteins using a standard nuclear expression plasmid. Expression was independent of other LCMV proteins but was blocked by a missense mutation within the original LCMV(WE) glycoprotein cDNA.
摘要
细胞质载体系统通常用于表达淋巴细胞性脉络丛脑膜炎病毒（LCMV）蛋白。然而，我们使用标准的核表达质粒实现了高水平的细胞表面糖蛋白表达。该表达不依赖于其他LCMV蛋白，但被原始LCMV（WE）糖蛋白cDNA内的一个错义突变所阻断。
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