Kay R, Alexander N J
Clin Exp Immunol. 1978 May;32(2):299-301.
Two washing procedures were used on spermatozoa to test the effect of centrifugation on immunofluorescent staining. The rapidly centrifuged spermatozoa often exhibited a loss of acrosomal but an increase in tail antigenicity. A standard technique of sperm preparation for immunofluorescence is recommended in order to have reliable and comparable results.
对精子采用了两种洗涤程序,以测试离心对免疫荧光染色的影响。快速离心的精子常常表现出顶体丧失,但尾部抗原性增加。为了获得可靠且可比的结果,推荐一种用于免疫荧光的精子制备标准技术。