Alexander N J, Kay R
Fertil Steril. 1977 Nov;28(11):1234-7.
Since cryopreservation of spermatozoa causes surface changes, we tested whether specific antigens associated with the plasmalemma might be dislodged or destroyed during freezing. When we compared the use of frozen sperm with the use of fresh sperm in indirect immunofluorescence tests for antisperm antibodies, we found changes in antigenicity. There was a decrease in antigenicity of the acrosomal region, and antigenicity of the sperm tail was increased with the addition of 10% glycerol, with or without cryopreservation. These results suggest that women who are infertile and, as demonstrated by immunofluorescence, have antibodies to sperm may be aided by artificial insemination with the husband's cryopreserved sperm.
由于精子的冷冻保存会导致表面变化，我们测试了与质膜相关的特定抗原在冷冻过程中是否可能被去除或破坏。当我们在抗精子抗体的间接免疫荧光试验中比较冷冻精子和新鲜精子的使用情况时，我们发现了抗原性的变化。顶体区域的抗原性降低，并且在添加10%甘油的情况下，无论是否进行冷冻保存，精子尾部的抗原性都会增加。这些结果表明，对于那些不育且经免疫荧光证明存在抗精子抗体的女性，使用丈夫的冷冻精子进行人工授精可能会有所帮助。
