Alexander N J
Andrologia. 1977 Apr-Jun;9(2):155-62. doi: 10.1111/j.1439-0272.1977.tb01275.x.
Samples of human semen frozen in liquid nitrogen ( - 196 degrees C) with no glycerol, 5 and 10% glycerol were compared with samples that were untreated, with 10% glycerol but not frozen, and spermatozoa frozen at -20 degrees C. SEM and TEM of the samples indicates that 10% glycerol caused fewer surface changes of the spermatozoa than other treatments. Motility counts after the various freezing treatments were also highest when 10% glycerol was used as the cryoprotectant. Nonetheless, cryopreservation is detrimental to spermatozoa and often causes considerable damage to the acrosome with a leakage of the acrosomal contents.
将人类精液样本分别在无甘油、含5%甘油和10%甘油的情况下于液氮（-196摄氏度）中冷冻，并与未经处理的样本、含10%甘油但未冷冻的样本以及在-20摄氏度下冷冻的精子进行比较。样本的扫描电子显微镜（SEM）和透射电子显微镜（TEM）分析表明，与其他处理方式相比，10%甘油导致精子表面变化较少。当使用10%甘油作为冷冻保护剂时，各种冷冻处理后的活力计数也最高。尽管如此，冷冻保存对精子是有害的，并且常常会对顶体造成相当大的损伤，导致顶体内容物泄漏。
