Department of Obstetrics and Gynecology, The first Hospital of Lanzhou University, Gansu, Lanzhou, China.
Bioengineered. 2022 Mar;13(3):7485-7499. doi: 10.1080/21655979.2022.2049026.
Endometrial cancer (EC) is one of the most common gynecological tumors with an increasing incidence. CircRNA plays an essential regulatory role in EC. Our objective was to investigate the potential mechanism of circRNAs derived SPOC Domain Containing 1 (SPOCD1) in EC progression. Seven circRNAs from SPOCD1 were analyzed by circBase and their expression was verified by quantitative real-time polymerase chain reaction. Only the expression of hsa_circ_0011324 was significantly increased in cancer tissues. The cell lines Ishikawa and RL95-2 which interfered with or overexpressed hsa_circ_0011324 were constructed and cell functions were tested. Results revealed hsa_circ_0011324 overexpression promoted cell proliferation, migration, and invasion; while silence of hsa_circ_0011324 had opposite effect on cell functions. RNA22 website and Targetscan website were applied to analyze downstream genes regulated by hsa_circ_0011324. Then, the expression of downstream genes was detected in EC tissues. Results indicated hsa-miR-497/16-5p expression were down-regulated, and mechanistic target of rapamycin kinase (mTOR) was up-regulated in EC. Furthermore, hsa_circ_0011324 regulated mTOR expression and cell functions by affecting hsa-miR-497/16-5p. And the potential mechanism was hsa_circ_0011324 competes with mTOR to directly bind to hsa-miR-497/16-5p. In conclusion, hsa_circ_0011324 could sponge hsa-miR-497/16-5p targeted mTOR to participate in EC progress. Our study may provide a new therapeutic target for EC.
子宫内膜癌（EC）是最常见的妇科肿瘤之一，发病率呈上升趋势。CircRNA 在 EC 中发挥着重要的调节作用。我们的目的是研究源自 SPOC 结构域包含 1（SPOCD1）的 circRNA 在 EC 进展中的潜在机制。通过 circBase 分析了 SPOCD1 中的 7 个 circRNA，并通过定量实时聚合酶链反应验证了它们的表达。只有 hsa_circ_0011324 的表达在癌组织中显著增加。构建了干扰或过表达 hsa_circ_0011324 的 Ishikawa 和 RL95-2 细胞系，并测试了细胞功能。结果表明 hsa_circ_0011324 的过表达促进了细胞增殖、迁移和侵袭；而 hsa_circ_0011324 的沉默对细胞功能则有相反的影响。应用 RNA22 网站和 Targetscan 网站分析了 hsa_circ_0011324 调控的下游基因。然后，检测了 EC 组织中下游基因的表达。结果表明，在 EC 中 hsa-miR-497/16-5p 的表达下调，而雷帕霉素靶蛋白激酶（mTOR）上调。此外，hsa_circ_0011324 通过影响 hsa-miR-497/16-5p 调节 mTOR 表达和细胞功能。潜在的机制是 hsa_circ_0011324 与 mTOR 竞争直接结合 hsa-miR-497/16-5p。总之，hsa_circ_0011324 可以通过海绵吸附 hsa-miR-497/16-5p 靶向 mTOR 参与 EC 进展。我们的研究可能为 EC 提供新的治疗靶点。
Zotero 插件
