Jia Keyu, Wang Shuchen, Dai Qi, Feng Yao, Zhang Qiuyi, Zhao Qianyu, Chen Fuxue, Li Zi, Xiao Ying, Zhong Yan, Zhu Zhenni, Wang Hongwei, Wang Junbo, Luo Zhong-Cheng, Zhou Hong, Gao Ying
CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.
School of Life Sciences, Shanghai University, Shanghai, China.
J Nutr. 2022 Jun 9;152(6):1507-1514. doi: 10.1093/jn/nxac054.
Trace elements may affect neurodevelopment. There is a lack of data on breast-milk rubidium (Rb) in relation to neurodevelopment in infants. The associations of copper (Cu), zinc (Zn) and strontium (Sr) with neurodevelopment in infants remain uncertain.
We sought to evaluate the associations of breast-milk Rb (primary exposure), Cu, Zn, and Sr with neurodevelopment in infants at age 8 months.
The study cohort included 117 breastfed infants. Breast-milk samples were collected at 42 days and 8 months postpartum. Breast-milk Rb, Zn, Cu, and Sr were measured by inductively coupled plasma mass spectrometer. Neurodevelopment was assessed at age 8 months. The primary outcomes were attention and working memory scores, as evaluated by the A-not-B task. Other outcomes included the Mental Development Index (MDI) and Psychomotor Development Index (PDI) as evaluated by the Bayley Scale of Infant Development III. Generalized linear models and restricted cubic spline regression were used to assess the associations between trace elements and neurodevelopment indices. Bonferroni correction was conducted on all data presented.
A nonlinear association was observed between breast-milk Rb at 42 days and infant's attention at age 8 months (nonlinearity P = 0.037). Positive associations were observed with infant MDI scores and breast-milk Rb at 42 days (β = 4.46; P = 0.06) and 8 months (β = 3.79; P = 0.009) postpartum. Breast-milk Zn at 42 days was positively associated with infant's attention (β = 0.31; P = 0.039). Sr at 42 days was positively correlated with attention (β = 0.18; P = 0.043) and MDI scores (β = 2.18; P = 0.015) at 8 months. Inverted U-shape associations were observed for breast-milk Cu at 42 days with infant attention and PDI scores. All associations were not significant after correction for multiple tests.
Our data suggest that Rb, Zn, Cu, and Sr in breast milk at certain concentrations are associated with neurodevelopment in breastfed infants. Further studies are warranted to validate the findings.
微量元素可能影响神经发育。关于母乳中的铷（Rb）与婴儿神经发育的关系，目前缺乏相关数据。铜（Cu）、锌（Zn）和锶（Sr）与婴儿神经发育的关联仍不明确。
我们试图评估母乳中的铷（主要暴露因素）、铜、锌和锶与8月龄婴儿神经发育之间的关联。
研究队列包括117名母乳喂养的婴儿。在产后42天和8个月时采集母乳样本。采用电感耦合等离子体质谱仪测定母乳中的铷、锌、铜和锶。在婴儿8个月大时评估其神经发育情况。主要结局指标是通过A非B任务评估的注意力和工作记忆得分。其他结局指标包括通过贝利婴儿发育量表第三版评估的心理发育指数（MDI）和精神运动发育指数（PDI）。使用广义线性模型和受限立方样条回归来评估微量元素与神经发育指标之间的关联。对所有呈现的数据进行了Bonferroni校正。
观察到产后42天母乳中的铷与8月龄婴儿的注意力之间存在非线性关联（非线性P = 0.037）。观察到产后42天（β = 4.46；P = 0.06）和8个月（β = 3.79；P = 0.009）时母乳中的铷与婴儿MDI得分呈正相关。产后42天母乳中的锌与婴儿注意力呈正相关（β = 0.31；P = 0.039）。产后42天的锶与8个月时的注意力（β = 0.18；P = 0.043）和MDI得分（β = 2.
