Hunan Key Laboratory of Biomedical Nanomaterials and Devices, College of Life Science and Chemistry, Hunan University of Technology, Zhuzhou 412007, China; State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China.
Hunan Key Laboratory of Biomedical Nanomaterials and Devices, College of Life Science and Chemistry, Hunan University of Technology, Zhuzhou 412007, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Jun 5;274:121097. doi: 10.1016/j.saa.2022.121097. Epub 2022 Mar 2.
Butyrylcholinesterase (BChE) is an enzyme which is relevant to a variety of diseases, and often serve as a common biomarker of health. In this work, a novel fluorescence sensor based on redox-regulated synthesis of polydopamine nanoparticles (PDANPs) has been developed for simple and sensitive sensing BChE activity. A facile and rapid one-step approach for the preparation of fluorescent PDANPs uses potassium permanganate to oxidize dopamine. We demonstrated that the fluorescence intensity of PDANPs is dependent on the dose of potassium permanganate. Butyrylcholinesterase catalyzes the hydrolysis of butyrylthiocholine iodide (BTCh) to produce thiolcholine (TCh) which in a redox reaction with potassium permanganate prevents the formation of fluorescent PDANP. As a result, the activity of BChE can be determined in line with changes in the fluorescence of PDANPs. Based on this finding, a convenient and label-free fluorescence sensor for BChE activity was established via redox-control of the fluorescence intensity of PDANPs. A dynamic response range for BChE is acquired within 0.5 ∼ 200 U/L along with a detection limit of 0.047 U/L. Importantly, the proposed method achieves practical application toward BChE in human sera. Moreover, its satisfying performance for screening of inhibitors was also proved. Hence, the proposed sensor holds great potential for cholinesterase-related biomedical investigation.
丁酰胆碱酯酶（BChE）是一种与多种疾病相关的酶，通常作为健康的常见生物标志物。在这项工作中，开发了一种基于氧化还原调控聚多巴胺纳米粒子（PDANPs）合成的新型荧光传感器，用于简单灵敏地检测 BChE 活性。使用高锰酸钾氧化多巴胺的简便快速的一步法制备了荧光 PDANPs。我们证明了 PDANPs 的荧光强度取决于高锰酸钾的剂量。丁酰胆碱酯酶催化丁酰硫代胆碱碘化物（BTCh）的水解，生成硫代胆碱（TCh），TCh 在与高锰酸钾的氧化还原反应中阻止了荧光 PDANP 的形成。因此，根据 PDANPs 荧光变化可以测定 BChE 的活性。基于这一发现，通过 PDANPs 荧光强度的氧化还原控制，建立了一种方便且无标记的 BChE 活性荧光传感器。BChE 的动态响应范围为 0.5~200 U/L，检测限为 0.047 U/L。重要的是，该方法在人血清中的 BChE 实际应用中取得了良好的效果。此外，还证明了其对抑制剂筛选的令人满意的性能。因此，该传感器在与胆碱酯酶相关的生物医学研究中具有很大的应用潜力。
