Shanghai Cancer Center and Department of Chemistry, Fudan University, Shanghai, People's Republic of China.
Institutes of Biomedical Sciences and NHC Key Laboratory of Glycoconjugates Research, Fudan University, Shanghai, People's Republic of China.
Rapid Commun Mass Spectrom. 2022 May 30;36(10):e9288. doi: 10.1002/rcm.9288.
Currently, cancer is one of the leading causes of death worldwide, partially owing to the lack of early diagnosis methods and effective therapies. With the rapid development of various omics, the precision medicine strategy becomes a promising way to increase the survival rates by considering individual differences. Glycosylation is one of the most essential protein post-translational modifications and plays important roles in a variety of biological processes. Therefore, it is highly possible to acquire understanding of the molecular mechanisms as well as discover novel potential markers for diagnosis and prognosis based on glycoproteomics research. This review summarizes the recent glycoproteomics studies about N-glycosylation of several cancer types, mainly in the past 5 years. We also highlight corresponding mass spectrometry-based analytical methods to give a brief overview on the main techniques applied in glycoproteomics.
目前，癌症是全球主要死因之一，部分原因是缺乏早期诊断方法和有效的治疗方法。随着各种组学的快速发展，精准医学策略通过考虑个体差异成为提高生存率的一种有前途的方法。糖基化是最重要的蛋白质翻译后修饰之一，在多种生物过程中发挥重要作用。因此，基于糖蛋白质组学研究，很有可能了解分子机制并发现新的潜在诊断和预后标志物。本综述总结了过去 5 年中几种癌症类型的 N-糖基化的最新糖蛋白质组学研究。我们还重点介绍了相应的基于质谱的分析方法，简要概述了糖蛋白质组学中应用的主要技术。
