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用于糖蛋白质组学的质谱策略

Mass Spectrometry Strategies for -Glycoproteomics.

作者信息

Helms Amanda, Brodbelt Jennifer S

机构信息

Department of Chemistry, The University of Texas at Austin, Austin, TX 78712, USA.

出版信息

Cells. 2024 Feb 25;13(5):394. doi: 10.3390/cells13050394.

Abstract

Glycoproteomics has accelerated in recent decades owing to numerous innovations in the analytical workflow. In particular, new mass spectrometry strategies have contributed to inroads in -glycoproteomics, a field that lags behind -glycoproteomics due to several unique challenges associated with the complexity of -glycosylation. This review will focus on progress in sample preparation, enrichment strategies, and MS/MS techniques for the identification and characterization of -glycoproteins.

摘要

近几十年来,由于分析流程中的众多创新,糖蛋白质组学得到了快速发展。特别是,新的质谱策略推动了O-糖蛋白质组学的进展,该领域由于与O-糖基化复杂性相关的几个独特挑战而落后于N-糖蛋白质组学。本综述将重点关注用于O-糖蛋白鉴定和表征的样品制备、富集策略及串联质谱技术的进展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/8c9c0435fb38/cells-13-00394-sch001.jpg

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