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用于糖蛋白质组学的质谱策略

Mass Spectrometry Strategies for -Glycoproteomics.

作者信息

Helms Amanda, Brodbelt Jennifer S

机构信息

Department of Chemistry, The University of Texas at Austin, Austin, TX 78712, USA.

出版信息

Cells. 2024 Feb 25;13(5):394. doi: 10.3390/cells13050394.

DOI:10.3390/cells13050394
PMID:38474358
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10930906/
Abstract

Glycoproteomics has accelerated in recent decades owing to numerous innovations in the analytical workflow. In particular, new mass spectrometry strategies have contributed to inroads in -glycoproteomics, a field that lags behind -glycoproteomics due to several unique challenges associated with the complexity of -glycosylation. This review will focus on progress in sample preparation, enrichment strategies, and MS/MS techniques for the identification and characterization of -glycoproteins.

摘要

近几十年来，由于分析流程中的众多创新，糖蛋白质组学得到了快速发展。特别是，新的质谱策略推动了O-糖蛋白质组学的进展，该领域由于与O-糖基化复杂性相关的几个独特挑战而落后于N-糖蛋白质组学。本综述将重点关注用于O-糖蛋白鉴定和表征的样品制备、富集策略及串联质谱技术的进展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/2d84eb2067d9/cells-13-00394-sch009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/8c9c0435fb38/cells-13-00394-sch001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/64008a59e02d/cells-13-00394-sch003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/608cfd9328de/cells-13-00394-sch004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/3487b855c201/cells-13-00394-sch005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/67050e919be5/cells-13-00394-sch006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/19838a57e469/cells-13-00394-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/242da435488f/cells-13-00394-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/8ebdf7f14603/cells-13-00394-sch007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/7b098e9768e4/cells-13-00394-sch008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/b8f19cbabee6/cells-13-00394-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/2d84eb2067d9/cells-13-00394-sch009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/8c9c0435fb38/cells-13-00394-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/8511a0f4cc08/cells-13-00394-sch002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/64008a59e02d/cells-13-00394-sch003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/608cfd9328de/cells-13-00394-sch004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/3487b855c201/cells-13-00394-sch005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/67050e919be5/cells-13-00394-sch006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/19838a57e469/cells-13-00394-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/242da435488f/cells-13-00394-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/8ebdf7f14603/cells-13-00394-sch007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/7b098e9768e4/cells-13-00394-sch008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/b8f19cbabee6/cells-13-00394-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/a75c6c4c0fe9/cells-13-00394-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b572/10930906/2d84eb2067d9/cells-13-00394-sch009.jpg

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