Department of Pharmaceutical Botany, Faculty of Pharmacy, Ataturk University, Erzurum, Turkey.
Ataturk University Medicinal and Aromatic Plant and Drug Research Center, Erzurum, Turkey.
Protoplasma. 2022 Nov;259(6):1493-1506. doi: 10.1007/s00709-022-01752-3. Epub 2022 Mar 9.
Colchicum speciosum Steven species is a perennial stemless plant. C. speciosum is a flowering herb native to mountainous regions of northern Turkey, the Caucasus, and northern Iran. It has been known as "Vargit, Acı Çiğdem, Güz Çiğdemi". The present study reports the antimicrobial, antioxidant, α-amylase and α-glucosidase inhibitory activities of corm, leaf and flower methanol extracts, anatomical (light and electron microscopes) properties of root, corm, leaf, flowers and morphological characteristics of C. speciosum. Three different part of extracts C. speciosum were evaluated against E. coli ATCC 8739, S. aureus ATCC 6538, B. subtilis ATCC 19,659, C. albicans ATCC 10,231, C. krusei ATCC 14,243, and C. tropicalis ATCC 750. The most effective extract was found to be MeOH extract for corm and leaf against C. tropicalis ATCC 750 strain with MIC value 160 > µg/mL. It has been investigated first time anatomy of the tepal, ovary, anther, filament of C. speciosum. Leaf extract was the highest phenolic component (78.61842 µg GAE/ mg extract). As a result of DPPH and ABTS tests, it was determined that the leaf extract showed the best activity (IC = 6.568 μg/mL and IC = 3.243 μg/mL, respectively). Corm extract exhibited α-glucosidase inhibitory activity with an IC value of 21039 µg/mL. This is the first study of the in vitro antimicrobial, antioxidant, antidiabetic activities, detailed anatomical and morphological properties of C. speciosum. HıGHLıGHTS : • Antioxidant-antidiabetic-antimicrobial potential of Colchicum speciosum • Leaf extract had the highest phenolic component • The leaf got the highest DPPH and ABTS antioxidant potential • Corm extract exhibited α-glucosidase inhibitory activity • The most effective extract was found to be MeOH extract for corm and leaf against C. tropicalis • This is the first study of the in vitro antimicrobial, antioxidant, antidiabetic activities, detailed anatomical and morphological properties of C. speciosum.
美丽雪花莲史蒂文物种是一种多年生无茎植物。C. speciosum 是一种原产于土耳其北部山区、高加索和伊朗北部的开花草本植物。它被称为“Vargit、Acı Çiğdem、Güz Çiğdemi”。本研究报告了块茎、叶和花甲醇提取物的抗菌、抗氧化、α-淀粉酶和α-葡萄糖苷酶抑制活性，根、块茎、叶、花的解剖学（光镜和电子显微镜）特性以及 C. speciosum 的形态特征。对三种不同部位的提取物 C. speciosum 进行了评估，以对抗大肠杆菌 ATCC 8739、金黄色葡萄球菌 ATCC 6538、枯草芽孢杆菌 ATCC 19659、白色念珠菌 ATCC 10231、克柔念珠菌 ATCC 14243 和热带念珠菌 ATCC 750。结果表明，MeOH 提取物对块茎和叶对热带念珠菌 ATCC 750 菌株的 MIC 值为 160>μg/mL，效果最佳。这是第一次对美丽雪花莲的花瓣、子房、花药、花丝进行解剖学研究。叶提取物是酚类成分含量最高的（78.61842μg GAE/mg 提取物）。根据 DPPH 和 ABTS 测试，发现叶提取物的活性最好（IC=6.568μg/mL 和 IC=3.243μg/mL）。块茎提取物对α-葡萄糖苷酶具有抑制活性，IC 值为 21039μg/mL。这是对美丽雪花莲的体外抗菌、抗氧化、抗糖尿病活性、详细的解剖学和形态学特性的首次研究。亮点：•美丽雪花莲的抗氧化-抗糖尿病-抗菌潜力•叶提取物含有最高的酚类成分•叶提取物具有最高的 DPPH 和 ABTS 抗氧化潜力•块茎提取物对α-葡萄糖苷酶具有抑制活性•对块茎和叶的 C. speciosum 甲醇提取物对 C. tropicalis 的抑制作用最有效•这是对美丽雪花莲的体外抗菌、抗氧化、抗糖尿病活性、详细的解剖学和形态学特性的首次研究。
