人甲状旁腺抗原：用单克隆抗体进行特性鉴定和定位
Human parathyroid antigen: characterization and localization with monoclonal antibodies.
作者信息
Cance W G, Wells S A, Dilley W G, Welch M J, Otsuka F L, Davie J M
出版信息
Proc Natl Acad Sci U S A. 1986 Aug;83(16):6112-6. doi: 10.1073/pnas.83.16.6112.
A cell surface antigen on human parathyroid cells was identified by monoclonal antibodies. The antigen, called parathyroid antigen (PTA), is found on both of two major polypeptides (190 kDa and 160 kDa) apparently associated exclusively with parathyroid cells. To determine whether PTA was a suitable target for in vivo imaging, 125I-labeled anti-PTA was injected into nude mice bearing human parathyroid xenografts. IgG1 anti-PTA antibody showed excellent radiolocalization of the grafts, whereas IgM anti-PTA, containing the same variable domains as the IgG1 antibody, showed little specific binding. These results suggest that antibody isotype is an important parameter for the in vivo immunoscintigraphy of specific cellular antigens.
通过单克隆抗体鉴定出了人甲状旁腺细胞上的一种细胞表面抗原。这种抗原被称为甲状旁腺抗原（PTA），在两种主要的多肽（190 kDa和160 kDa）上均有发现，这两种多肽显然仅与甲状旁腺细胞相关。为了确定PTA是否是体内成像的合适靶点，将125I标记的抗PTA注入携带人甲状旁腺异种移植物的裸鼠体内。IgG1抗PTA抗体对移植物显示出出色的放射性定位，而含有与IgG1抗体相同可变区的IgM抗PTA则几乎没有特异性结合。这些结果表明，抗体亚型是特异性细胞抗原体内免疫闪烁成像的一个重要参数。
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