多种循环脂质成分中的脂肪酸反映了人类肝脏甘油三酯的组成。

Fatty acids in multiple circulating lipid fractions reflects the composition of liver triglycerides in humans.

机构信息

Dept. of Public Health and Caring Sciences, Clinical Nutrition and Metabolism, Uppsala University, Uppsala, Sweden.

Dept. of Medical Sciences, Section of Gastroenterology and Hepatology, Uppsala University, Uppsala, Sweden.

出版信息

Clin Nutr. 2022 Apr;41(4):805-809. doi: 10.1016/j.clnu.2022.02.005. Epub 2022 Feb 11.

DOI:10.1016/j.clnu.2022.02.005

PMID:35263689

Abstract

BACKGROUND AND AIMS

Fatty acids (e.g. 16:1n-7) and desaturase indices (e.g. stearoyl-CoA desaturase, SCD) in plasma cholesteryl esters (CE) and phospholipids (PL) are used as biomarkers of dietary fat quality and lipid metabolism and are associated with disease outcomes. Endogenously produced circulating fatty acids are believed to reflect composition of the liver, yet little data exist to support such relationship. We investigated associations between circulating fatty acids and fatty acids within the liver.

METHODS

Liver biopsies and blood were collected from n = 60 patients with non-alcoholic fatty liver disease. Fatty acids in CE, PL and triglycerides (TG) in plasma and liver were analyzed using gas chromatography. Associations were assessed using Spearman rank correlations.

RESULTS

Overall, fatty acids and desaturase indices in plasma PL and TG showed moderate-strong correlations with fatty acids and desaturase indices in corresponding lipid fractions in liver. For plasma CE, 16:1n-7 and SCD were correlated with 16:1n-7 and SCD in liver CE. Noteworthy, fatty acids in plasma CE and PL also showed moderate-strong correlations with fatty acids in liver TG (e.g. r = 0.82-0.87 for 16:1n-7 and r = 0.77 for SCD).

CONCLUSION

We demonstrate that fatty acids in circulating lipid fractions, including CE, TG and PL, reflects the composition of liver TG in humans, suggesting that circulating fatty acids might be useful biomarkers for the fatty acid composition of the liver. As liver tissue is rarely available in cohort studies, our findings could enhance our understanding of plasma fatty acids as markers of hepatic lipid metabolism and their links to metabolic diseases.

摘要

背景与目的

血浆胆固醇酯（CE）和磷脂（PL）中的脂肪酸（如 16:1n-7）和去饱和酶指数（如硬脂酰辅酶 A 去饱和酶，SCD）可用作膳食脂肪质量和脂质代谢的生物标志物，并与疾病结局相关。内源性产生的循环脂肪酸被认为反映了肝脏的组成，但支持这种关系的数据很少。我们研究了循环脂肪酸与肝脏内脂肪酸之间的关系。

方法

从 60 名非酒精性脂肪性肝病患者中采集肝活检和血液。使用气相色谱法分析血浆 CE 和 PL 中的脂肪酸以及肝脏中的甘油三酯（TG）中的脂肪酸。使用 Spearman 秩相关评估相关性。

结果

总体而言，血浆 PL 和 TG 中的脂肪酸和去饱和酶指数与肝脏相应脂质部分中的脂肪酸和去饱和酶指数呈中强相关。对于血浆 CE，16:1n-7 和 SCD 与肝脏 CE 中的 16:1n-7 和 SCD 相关。值得注意的是，血浆 CE 和 PL 中的脂肪酸与肝脏 TG 中的脂肪酸也呈中强相关（例如，16:1n-7 的 r 值为 0.82-0.87，SCD 的 r 值为 0.77）。