School of Chemistry and Biological Engineering, University of Science & Technology Beijing, 0 Xueyuan Road, Beijing, 100083, People's Republic of China.
Marshall Laboratory of Biomedical Engineering, Research Center for Biosensor and Nanotheranostic, School of Biomedical Engineering, Shenzhen University, Guangdong, 518060, People's Republic of China.
J Nanobiotechnology. 2022 Mar 9;20(1):121. doi: 10.1186/s12951-022-01331-x.
Optically active nanostructures consisting of organic compounds and metallic support have shown great promise in phototherapy due to their increased light absorption capacity and high energy conversion. Herein, we conjugated chlorophyll (Chl) to vanadium carbide (VC) nanosheets for combined photodynamic/photothermal therapy (PDT/PTT), which reserves the advantages of each modality while minimizing the side effects to achieve an improved therapeutic effect. In this system, the Chl from Leptolyngbya JSC-1 extracts acted as an efficient light-harvest antenna in a wide NIR range and photosensitizers (PSs) for oxygen self-generation hypoxia-relief PDT. The available large surface of two-dimensional (2D) VC showed high Chl loading efficiency, and the interaction between organic Chl and metallic VC led to energy conversion efficiency high to 78%. Thus, the Chl/ VC nanostructure showed advanced performance in vitro cell line killing and completely ablated tumors in vivo with 100% survival rate under a single NIR irradiation. Our results suggest that the artificial optical Chl/VC nanostructure will benefit photocatalytic tumor eradication clinic application.
由有机化合物和金属载体组成的手性纳米结构由于其增加的光吸收能力和高能量转换而在光疗中显示出巨大的应用前景。在此，我们将叶绿素（Chl）共轭到碳化钒（VC）纳米片中用于联合光动力/光热治疗（PDT/PTT），保留了每种模式的优点，同时最小化副作用，以实现改善的治疗效果。在该系统中，从 Leptolyngbya JSC-1 提取物中提取的叶绿素作为高效的宽近红外范围的光收集天线和用于氧气自产生缓解缺氧 PDT 的光敏剂（PS）。二维（2D）VC 的可用大表面表现出高的叶绿素负载效率，并且有机叶绿素和金属 VC 之间的相互作用导致能量转换效率高达 78％。因此，Chl/VC 纳米结构在体外细胞系杀伤中表现出先进的性能，并在单次近红外照射下体内完全消融肿瘤，存活率为 100％。我们的结果表明，人工光学 Chl/VC 纳米结构将有益于光催化肿瘤消除的临床应用。
