Department of Experimental Animals, Interdisciplinary Center for Science Research, Head Office for Research and Academic Information, Shimane University, 89-1 Enya-cho, Izumo, Shimane 693-8501, Japan.
Department of Biosignaling and Radioisotope Experiment, Interdisciplinary Center for Science Research, Head Office for Research and Academic Information, Shimane University, 89-1 Enya-cho, Izumo, Shimane 693-8501, Japan.
Exp Anim. 2022 Aug 5;71(3):347-355. doi: 10.1538/expanim.21-0187. Epub 2022 Mar 10.
Hydrogen-rich water (HW) has been suggested to possess antioxidant properties of value in treatments of lifestyle diseases and for prevention of latent pathologies. To date, the potential benefits of HW against the deleterious effects of excessive salt intake and hypertension have not been investigated. Here, we first examined the effects of HW or HW supplemented with 0.1% ascorbic acid (HWA) on spontaneously hypertensive rats (SHR) that had been fed a normal diet. In comparison to control rats given distilled water (DW), we found that HW did not significantly influence systolic blood pressure (SBP) or diastolic blood pressure (DBP) in SHR; however, the increase in SBP and DBP were inhibited in the HWA group. Next, four groups of SHR were given DW, 0.1% ascorbic acid-added DW (DWA), HW, or HWA in combination with a 4% NaCl-added diet. SHR fed the 4% NaCl-added diet showed increased hypertension; HWA treatment resulted in a significant reduction in blood pressure. The HWA group tended to have lower plasma angiotensin II levels than the DW group. In addition, urinary volumes and urinary sodium levels were significantly lower in the HWA group than the DW group. Urinary isoprostane, an oxidative stress marker, was also significantly lower in the HWA group, suggesting that the inhibitory effect of HWA on blood pressure elevation was caused by a reduction in oxidative stress. These findings suggest a synergistic interaction between HW and ascorbic acid, and also suggest that HWA ingestion has potential for prevention of hypertension.
富氢水（HW）被认为具有抗氧化特性，可用于治疗生活方式疾病和预防潜在的病理变化。迄今为止，HW 对过量盐摄入和高血压的有害影响的潜在益处尚未得到研究。在这里，我们首先研究了 HW 或补充了 0.1%抗坏血酸（HWA）的 HW 对正常饮食的自发性高血压大鼠（SHR）的影响。与给予蒸馏水（DW）的对照组大鼠相比，我们发现 HW 对 SHR 的收缩压（SBP）或舒张压（DBP）没有显著影响；然而，HWA 组抑制了 SBP 和 DBP 的增加。接下来，将四组 SHR 分别给予 DW、添加 0.1%抗坏血酸的 DW（DWA）、HW 或 HWA 与添加 4%NaCl 的饮食。给予 4%NaCl 饮食的 SHR 表现出高血压增加；HWA 治疗导致血压显著降低。HWA 组的血压比 DW 组低。此外，HWA 组的血浆血管紧张素 II 水平低于 DW 组，尿体积和尿钠水平也显著低于 DW 组。尿液中氧化应激标志物 8-异前列腺素也显著低于 DW 组，这表明 HWA 对血压升高的抑制作用是由氧化应激减少引起的。这些发现表明 HW 和抗坏血酸之间存在协同相互作用，并且 HWA 摄入具有预防高血压的潜力。
