埃塞俄比亚亚的斯亚贝巴梅内利克二世转诊医院外眼感染患者的抗生素敏感性模式及细菌谱
Antibiotic Susceptibility Pattern and Bacterial Spectrum Among Patients with External Eye Infections at Menelik II Referral Hospital in Addis Ababa, Ethiopia.
作者信息
Woreta Asmamaw Nitsuh, Kebede Habtamu Biazin, Tilahun Yonas, Teklegiorgis Solomon Gebreselassie, Abegaz Woldaregay Erku
机构信息
Department of Microbiology, Immunology and Parasitology, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia.
Microbiology Unit, Department of Laboratory Sciences, Menelik II Referral Hospital, Addis Ababa, Ethiopia.
出版信息
Infect Drug Resist. 2022 Mar 3;15:765-779. doi: 10.2147/IDR.S352098. eCollection 2022.
BACKGROUND
Eye infections can cause loss or impairment of visual function and can lead to severe impairment. Bacteria are the most common pathogens that affect the structure of the eye. As a result, quick identification of the causative agents and testing of their medication susceptibility are essential for effective treatment of eye infections. This study was intended for determining the extent of bacterial isolates from external eye infections (EEIs) and their susceptibility to antibiotics.
METHODS
A facility-based cross-sectional study was conducted among patients attending Menelik II Referral Hospital. The study comprised patients who had EEIs verified. EEI samples were collected using sterile methods. Bacterial isolates were identified using gram stain, colony morphology, and biochemical tests. The Kirby-Bauer disk diffusion technique was used to conduct a drug susceptibility test.
RESULTS
Totally, 323 participants were recruited for this study and 184 bacterial isolates were obtained from 175 (54.5%) participants. The main clinical diagnosis was blepharitis 122 (37.8%), followed by conjunctivitis 73 (22.6%) and keratitis 57 (17.6%). The gram-positive isolates were 171 (92.9%). CoNS with a frequency of 76 (41.3%) was the most common bacterial isolates, followed by 67 (36.4%), 16 (8.7%), and species 6(3.3%). Gram-positive isolates were sensitive to tobramycin, gentamicin, chloramphenicol, vancomycin, and ceftriaxone. In contrast, 94.0% of these gram-positive isolates showed resistance to penicillin. Multidrug resistance (MDR) was observed in both gram-positive and negative bacteria at rates of 123 (72%) and 12 (92.1%), respectively. The overall MDR rate among the isolates was 135 (73.4%).
CONCLUSION
In this study, blepharitis was the major EEI, followed by conjunctivitis. The predominant bacterial species isolated from EEIs were CoNS, followed by . More than half of the isolates were drug-resistant, with a large number being multidrug-resistant, highlighting the necessity for continued and coordinated surveillance to hunt for infections that are known to be resistant.
背景
眼部感染可导致视力功能丧失或受损，并可能导致严重损害。细菌是影响眼部结构的最常见病原体。因此，快速鉴定病原体并检测其药物敏感性对于有效治疗眼部感染至关重要。本研究旨在确定外眼感染（EEIs）中细菌分离株的程度及其对抗生素的敏感性。
方法
在梅内利克二世转诊医院就诊的患者中进行了一项基于机构的横断面研究。该研究纳入了经证实患有EEIs的患者。使用无菌方法采集EEI样本。通过革兰氏染色、菌落形态和生化试验鉴定细菌分离株。采用 Kirby-Bauer 纸片扩散技术进行药敏试验。
结果
本研究共招募了323名参与者，从175名（54.5%）参与者中获得了184株细菌分离株。主要临床诊断为睑缘炎122例（37.8%），其次是结膜炎73例（22.6%）和角膜炎57例（17.6%）。革兰氏阳性分离株有171株（92.9%）。凝固酶阴性葡萄球菌（CoNS）出现频率为76株（41.3%），是最常见的细菌分离株，其次是[此处原文缺失部分内容]67株（36.4%）、[此处原文缺失部分内容]16株（8.7%）和[此处原文缺失部分内容]菌种6株（3.3%）。革兰氏阳性分离株对妥布霉素、庆大霉素、氯霉素、万古霉素和头孢曲松敏感。相比之下，这些革兰氏阳性分离株中有94.0%对青霉素耐药。在革兰氏阳性和阴性细菌中均观察到多重耐药（MDR），发生率分别为123例（72%）和12例（92.1%）。分离株中的总体MDR率为135例（73.4%）。
结论
在本研究中，睑缘炎是主要的EEI，其次是结膜炎。从EEIs中分离出的主要细菌种类是CoNS，其次是[此处原文缺失部分内容]。超过一半的分离株耐药，其中大量为多重耐药，这突出表明需要持续和协调的监测，以寻找已知耐药的感染病例。
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