眼部感染中的细菌病原体和抗菌药物敏感性：埃塞俄比亚德西博鲁-梅达综合医院的一项研究。

Bacterial pathogens and antimicrobial susceptibility in ocular infections: A study at Boru-Meda General Hospital, Dessie, Ethiopia.

机构信息

Boru-meda General Hospital, Dessie, Ethiopia.

Department of Medical Laboratory Science, College of Medicine and Health Sciences, Wollo University, P.O. Box 1145, Dessie, Ethiopia.

出版信息

BMC Ophthalmol. 2024 Aug 13;24(1):342. doi: 10.1186/s12886-024-03544-0.

DOI:10.1186/s12886-024-03544-0

PMID:39138386

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11323621/

Abstract

INTRODUCTION

The eye consists of both internal and external compartments. Several variables, including microbes, dust, and high temperatures can cause eye illnesses that can result in blindness. Bacterial eye infections continue to be a major cause of ocular morbidity and blindness, and their prevalence is periodically rising. The objective of the study was to detect bacterial pathogens and assess their susceptibility profiles to antibiotics in the ophthalmology unit of Boru-meda Hospital in Dessie, Ethiopia.

METHODS

A hospital-based cross-sectional study was conducted from February 1 to April 30, 2021, among 319 study participants with symptomatic ocular or peri-ocular infections who were enrolled using a consecutive sampling technique. After proper specimen collection, the specimen was immediately inoculated with chocolate, blood, and MacConkey agar. After pure colonies were obtained, they were identified using standard microbiological methods. The Kirby Bauer disk diffusion method was used to test antimicrobial susceptibility patterns, based on the guidelines of the Clinical and Laboratory Standards Institute.

RESULTS

The majority of participants developed conjunctivitis 126 (39.5%), followed by blepharitis 47 (14.73%), and dacryocystitis 45 (14.1%). Overall, 164 (51.4%) participants were culture positive, six (1.9%) participants had mixed bacterial isolates, giving a total of 170 bacterial isolates with an isolation rate of 53.3%. The predominant species was CoNS 47 (27.6%), followed by S. aureus 38 (22.4%) and Moraxella species 32 (18.8%). The overall Multi-Drug Resistance (MDR) rate was 62.9%, with 33 (44.6%) being gram-negative and 74 (77.1%) being gram-positive isolates.

CONCLUSION

Conjunctivitis was the dominant clinical case and CoNS, was the predominant isolate. A higher rate of MDR isolates, particularly gram-positive ones, was observed. Efficient peri-ocular or ocular bacterial infection surveillance, including microbiological laboratory data, is necessary for monitoring disease trends.

摘要

引言

眼睛包括内部和外部两个部分。微生物、灰尘和高温等多种因素都可能导致眼部疾病，从而导致失明。细菌性眼部感染仍然是眼部发病率和失明的主要原因，其流行率在不断上升。本研究的目的是在埃塞俄比亚德西市 Boru-meda 医院的眼科病房检测细菌病原体，并评估其对抗生素的敏感性。

方法

这是一项于 2021 年 2 月 1 日至 4 月 30 日在 319 名有眼部或眼周感染症状的研究参与者中进行的基于医院的横断面研究，使用连续抽样技术纳入参与者。标本采集后，立即用巧克力、血液和 MacConkey 琼脂接种。获得纯菌落后，采用标准微生物学方法进行鉴定。根据临床和实验室标准协会的指南，采用 Kirby Bauer 纸片扩散法检测抗生素药敏模式。

结果

大多数参与者发生结膜炎 126 例（39.5%），其次是睑缘炎 47 例（14.73%）和泪囊炎 45 例（14.1%）。总体而言，164 例（51.4%）参与者的培养结果为阳性，6 例（1.9%）参与者有混合细菌分离株，共分离出 170 株细菌，分离率为 53.3%。主要分离株为凝固酶阴性葡萄球菌 47 株（27.6%），其次是金黄色葡萄球菌 38 株（22.4%）和莫拉菌属 32 株（18.8%）。总体而言，多药耐药率为 62.9%，其中革兰氏阴性菌 33 株（44.6%），革兰氏阳性菌 74 株（77.1%）。