Bouhia Youness, Hafidi Mohamed, Ouhdouch Yedir, El Boukhari Mohammed El Mehdi, El Fels Loubna, Zeroual Youssef, Lyamlouli Karim
Laboratory of Microbial Biotechnology, Agrosciences and Environment, Labelled Research Unit-CNRST N°4, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakesh, Morocco.
Biodiversity and Plant Sciences Program, AgroBioSciences Department, Mohammed VI Polytechnic University (UM6P), Benguerir, Morocco.
Front Microbiol. 2022 Feb 21;12:814553. doi: 10.3389/fmicb.2021.814553. eCollection 2021.
Olive mill wastewater sludge (OMWS) is the main by-product of the olive industry. OMWS is usually dumped in landfills without prior treatment and may cause several eco-environmental hazards due to its high toxicity, which is mainly attributed to polyphenols and lipids. OMWS is rich in valuable biocompounds, which makes it highly desirable for valorization by composting. However, there is a need to understand how microbial communities evolve during OMWS composting with respect to physicochemical changes and the dynamics of pollutant degradation. In this study, we addressed the relationship between microbial community, physicochemical variations and pollutants degradation during the co-composting of OMWS and green wastes using metagenomic- and culture-dependent approaches. The results showed that in raw OMWS, was the most represented genus with almost 53% of the total identified fungal population. Moreover, the bacteria that dominated were (20%) and sp. (19%). The addition of green waste to OMWS improved the actinobacterial diversity of the mixture and enhanced the degradation of lipids (81.3%) and polyphenols (84.54%). Correlation analysis revealed that Actinobacteria and fungi ( sp., sp., and were the microorganisms that had the greatest influence on the composting process. Overall, these findings provide for the first time some novel insights into the microbial dynamics during OMWS composting and may contribute to the development of tailored inoculum for process optimization.
橄榄油厂废水污泥（OMWS）是橄榄油产业的主要副产品。OMWS通常未经预处理就被倾倒在垃圾填埋场，由于其高毒性（主要归因于多酚和脂质），可能会造成多种生态环境危害。OMWS富含宝贵的生物化合物，这使得通过堆肥进行增值利用非常具有吸引力。然而，有必要了解在OMWS堆肥过程中微生物群落如何随着物理化学变化和污染物降解动态而演变。在本研究中，我们使用宏基因组学和基于培养的方法，研究了OMWS与绿色废物共堆肥过程中微生物群落、物理化学变化和污染物降解之间的关系。结果表明，在未处理的OMWS中，是最主要的属，占已鉴定真菌总数的近53%。此外，占主导地位的细菌是（20%）和sp.（19%）。向OMWS中添加绿色废物提高了混合物中放线菌的多样性，并增强了脂质（81.3%）和多酚（84.54%）的降解。相关性分析表明，放线菌和真菌（sp.、sp.和是对堆肥过程影响最大的微生物。总体而言，这些发现首次为OMWS堆肥过程中的微生物动态提供了一些新见解，并可能有助于开发用于工艺优化的定制接种物。
