Clinical Laboratory, First Affiliated Hospital of Harbin Medical University, 150001, China.
Clinical Laboratory, Qingdao Hospital of Traditional Chinese Medicine (Qingdao Hiser Hospital), 266033, China.
Comput Math Methods Med. 2022 Feb 27;2022:4446016. doi: 10.1155/2022/4446016. eCollection 2022.
Systemic lupus erythematosus (SLE) is an autoimmune disease that can cause damage to multiple systems of the body. A number of studies have shown that long-chain noncoding RNA (lncRNA) can participate in the occurrence and development of a variety of autoimmune diseases. This study is aimed at detecting the expression levels of 5 lncRNAs in SLE patients and healthy controls and at exploring the relationship between expression levels and clinical symptoms and laboratory indicators.
The design type of this study is a case-control study. A total of 76 SLE patients and 71 healthy controls were included in the first phase of the study. Real-time fluorescence quantitative polymerase chain reaction was used to detect the expression level of 5 kinds of lncRNAs including lnc7514, lnc0640, lncagf, nc3643, and lnc5150 in PBMCs of two groups of patients; the expression of lncRNAs in the case group and the control group was analyzed. We analyzed the differences in the expression levels of lncRNAs between case and control groups, and explored the association of expression levels with clinical manifestations and laboratory characteristics. SPSS23.0 was used to analyze the expression level and gene polymorphism results; the statistical analysis test level = 0.05.
The expression level of lnc0640 in PBMCs of SLE patient group was higher than that of healthy control group ( = -3.56, = 0.03). However, lnc5150 was lower than in healthy controls ( = -7.16, < 0.001). lnc3643 expression levels were lower in SLE patients of SLE patients with pleurisy was lower than that of patients without pleurisy ( = -2.44, = 0.02). Low lnc3643 expression levels were observed in PBMCs with SLE patients with rash symptoms ( = -2.75, = 0.013). SLE expressed lower lnc3643 levels in PBMCs with SLE compared with those without pleurisy ( = -2.42, = 0.02). The above differences were statistically significant. Association analysis of lncRNA expression levels and clinical manifestations in SLE patients found that SLE was lower than those without rash or pleurisy (both < 0.05); association analysis of lncRNA expression level and laboratory results found a negative correlation between lnc3643, lnc7514, and SLE disease activity score (SLEDAI-2K), blood sink (ESR), and C-reactive protein (CRP) (all < 0.05).
lnc0640 was overexpressed in PBMCs in SLE patients compared with healthy controls. lnc3643 was negatively correlated with SLEDAI, and expression levels were associated with SLE patients with arthritis, rash, and pleuritis.
系统性红斑狼疮（SLE）是一种自身免疫性疾病，可导致机体多系统损伤。多项研究表明，长链非编码 RNA（lncRNA）可参与多种自身免疫性疾病的发生发展。本研究旨在检测 SLE 患者和健康对照者中 5 种 lncRNA 的表达水平，并探讨其表达水平与临床症状和实验室指标的关系。
本研究设计类型为病例对照研究。共纳入 76 例 SLE 患者和 71 例健康对照者纳入研究的第一阶段。采用实时荧光定量聚合酶链反应检测两组患者外周血单个核细胞（PBMCs）中 5 种 lncRNA（lnc7514、lnc0640、lncagf、nc3643 和 lnc5150）的表达水平；分析病例组和对照组 lncRNA 的表达差异，探讨表达水平与临床表现和实验室特征的关系。采用 SPSS23.0 对表达水平和基因多态性结果进行分析；检验水准 = 0.05。
SLE 患者 PBMCs 中 lnc0640 的表达水平高于健康对照组（ = -3.56， = 0.03）。而 lnc5150 则低于健康对照组（ = -7.16， < 0.001）。SLE 患者 PBMCs 中 lnc3643 的表达水平在合并胸膜炎患者中低于无胸膜炎患者（ = -2.44， = 0.02）。SLE 患者中合并皮疹症状者 PBMCs 中 lnc3643 的表达水平低于无皮疹者（ = -2.75， = 0.013）。SLE 患者中合并胸膜炎者 PBMCs 中 lnc3643 的表达水平低于无胸膜炎者（ = -2.42， = 0.02）。上述差异均有统计学意义。SLE 患者 lncRNA 表达水平与临床表现的关联分析发现，与无皮疹或胸膜炎者相比，SLE 患者的 lnc3643 表达水平更低（均 < 0.05）；lncRNA 表达水平与实验室结果的关联分析发现，lnc3643、lnc7514 与 SLE 疾病活动评分（SLEDAI-2K）、血沉降率（ESR）和 C 反应蛋白（CRP）呈负相关（均 < 0.05）。
与健康对照组相比，SLE 患者 PBMCs 中 lnc0640 呈过表达，lnc3643 与 SLEDAI 呈负相关，其表达水平与关节炎、皮疹和胸膜炎的 SLE 患者相关。
