HK2 基因表达与肾细胞癌发生、免疫细胞浸润及预后的相关性。
The Correlation of HK2 Gene Expression with the Occurrence, Immune Cell Infiltration, and Prognosis of Renal Cell Carcinoma.
机构信息
School of Clinical Medicine, Henan University of Science and Technology, The First Affiliated Hospital of Henan University of Science and Technology, China.
出版信息
Dis Markers. 2022 Feb 27;2022:1452861. doi: 10.1155/2022/1452861. eCollection 2022.
OBJECTIVES
Hexokinase 2 (HK2) is one of the key factors involved in the development of several human cancers. However, its role in immune cell infiltration (ICI) and tumor development in renal cell carcinoma is not yet known. Thus, we aimed to explore its relationship with ICI, overall survival, and prognosis of renal cell carcinoma.
METHODS
In this study, RNA-seq data from renal cancer and normal tissues were extracted from TCGA and the relationship between HK2 expression and pathological features of RCC patients was analyzed using the GEPIA and UALCAN databases. Subsequently, Western blot and qRT-PCR were performed to analyze the protein and mRNA expression of HK2 in renal cell carcinoma tissues and cell lines. Lastly, various bioinformatics tools were applied to determine the immune cell infiltration, survival, and developing prediction model.
RESULTS
The analysis of RNA-seq data revealed a high expression of HK2 in renal cell carcinoma; furthermore, Western blot and qRT-PCR also showed high expression of HK2 in renal cancer tissues and cell lines. The high expression of HK2 showed a significant positive correlation with the advanced stage of the tumor, lymph node metastasis, and worst survival in renal carcinoma patients. The high expression of HK2 was further identified as an independent risk factor of RCC patients; it also showed a significant positive immune cell infiltration RCC tumor microenvironment including macrophages, B cells, neutrophils, dendritic cells, and CD8 T cells.
CONCLUSION
the expression of HK2 is positively correlated with the immune cell infiltration and prognosis of renal cell carcinoma patients, thus playing an important role in renal cancer development.
目的
己糖激酶 2（HK2）是参与多种人类癌症发展的关键因素之一。然而，其在肾细胞癌中免疫细胞浸润（ICI）和肿瘤发展中的作用尚不清楚。因此，我们旨在探讨其与肾细胞癌的 ICI、总生存率和预后的关系。
方法
本研究从 TCGA 中提取了肾肿瘤和正常组织的 RNA-seq 数据，并使用 GEPIA 和 UALCAN 数据库分析了 HK2 表达与 RCC 患者病理特征的关系。随后，通过 Western blot 和 qRT-PCR 分析了肾细胞癌组织和细胞系中 HK2 的蛋白和 mRNA 表达。最后，应用各种生物信息学工具来确定免疫细胞浸润、生存和开发预测模型。
结果
RNA-seq 数据分析显示 HK2 在肾细胞癌中高表达；此外，Western blot 和 qRT-PCR 也显示 HK2 在肾癌细胞和组织中高表达。HK2 的高表达与肿瘤的晚期、淋巴结转移和肾细胞癌患者的最差生存呈显著正相关。HK2 的高表达进一步被确定为 RCC 患者的独立危险因素；它还显示出与肾癌细胞肿瘤微环境中巨噬细胞、B 细胞、中性粒细胞、树突状细胞和 CD8 T 细胞等免疫细胞浸润的显著正相关。
结论
HK2 的表达与肾细胞癌患者的免疫细胞浸润和预后呈正相关，因此在肾癌的发展中发挥着重要作用。
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