Mahallawi Waleed, Khabour Omar F, Al-Saedi Abdullah, Almuzaini Ziyad, Ibrahim Nadir
Medical Laboratory Technology Department, College of Applied Medical Sciences, Taibah University, Madinah, SAU.
Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, Jordan University of Science and Technology, Irbid, JOR.
Cureus. 2022 Feb 3;14(2):e21860. doi: 10.7759/cureus.21860. eCollection 2022 Feb.
Background and objective Human cytomegalovirus (HCMV), a double-stranded DNA virus of the family, can remain latent for long periods of time. HCMV may cause severe illness in immunocompromised patients and is associated with congenital anomalies. This study aimed to determine the anti-HCMV immunoglobulin G (IgG) and IgM antibody seroprevalence among blood-donating Saudi men in the Madinah region. Methods A total of 375 blood-donating Saudi men were recruited from the Central Blood Bank in Madinah, the Kingdom of Saudi Arabia (KSA), and stratified into three age groups: 18-30, 31-40, and 41-61 years. Anti-HCMV IgG and IgM antibody levels were measured by enzyme-linked immunosorbent assay (ELISA). Pearson's correlation coefficient was used to correlate antibody levels with variables. Results Most of the tested samples (95.73%, n=356) were positive for anti-HCMV IgG antibodies, but only 1.6% (n=6) were positive for both IgM and IgG antibodies, and all of them belonged to the age groups of 31-40 and 41-61 years. A strong inverse correlation was found between anti-HCMV IgG antibody levels and age (r=-0.51, p<0.0001). Additionally, there was an inverse correlation between anti-HCMV IgG antibody levels and body mass index (BMI) (r=-0.11, =0.036). No correlations were found between anti-HCMV IgG levels and hemoglobin levels or blood groups of the participants. Conclusions Blood-donating Saudi men in Madinah had a high seroprevalence of anti-HCMV IgG antibodies, indicating previous viral exposure. Age and BMI might influence the humoral immunologic memory response against HCMV, which appears to be endemic in Madinah.
背景与目的 人巨细胞病毒（HCMV）是疱疹病毒科的一种双链DNA病毒，可长期潜伏。HCMV可导致免疫功能低下患者患上严重疾病，并与先天性异常有关。本研究旨在确定沙特麦地那地区献血男性中抗HCMV免疫球蛋白G（IgG）和IgM抗体的血清流行率。方法 从沙特阿拉伯王国（KSA）麦地那中央血库招募了375名沙特献血男性，并将其分为三个年龄组：18 - 30岁、31 - 40岁和41 - 61岁。采用酶联免疫吸附测定（ELISA）法检测抗HCMV IgG和IgM抗体水平。使用Pearson相关系数将抗体水平与变量进行关联。结果 大多数检测样本（95.73%，n = 356）抗HCMV IgG抗体呈阳性，但只有1.6%（n = 6）的样本IgM和IgG抗体均呈阳性，且所有这些样本均属于31 - 40岁和41 - 61岁年龄组。发现抗HCMV IgG抗体水平与年龄之间存在强烈的负相关（r = -0.51，p < 0.0001）。此外，抗HCMV IgG抗体水平与体重指数（BMI）之间也存在负相关（r = -0.11，p = 0.036）。未发现抗HCMV IgG水平与参与者的血红蛋白水平或血型之间存在相关性。结论 麦地那的沙特献血男性中抗HCMV IgG抗体的血清流行率较高，表明既往有病毒暴露史。年龄和BMI可能会影响针对HCMV的体液免疫记忆反应，HCMV在麦地那似乎呈地方性流行。
