Alderson A, Sabelli P A, Dickinson J R, Cole D, Richardson M, Kreis M, Shewry P R, Halford N G
Department of Biochemistry and Physiology, Institute of Arable Crops Research Harpenden, Hertfordshire, United Kingdom.
Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8602-5. doi: 10.1073/pnas.88.19.8602.
A cDNA, cRKIN1, encoding a putative homologue of the yeast (Saccharomyces cerevisiae) SNF1-encoded protein-serine/threonine kinase, has been isolated from a library prepared from rye endosperm mRNA. Northern blot analysis demonstrated the presence of cRKIN1-related transcripts in developing endosperms but not in shoots, and Southern blot analysis showed the presence of a small gene family. SNF1 plays a central role in carbon catabolite repression in yeast and expression of the RKIN1 sequence in yeast snf1 mutants restored SNF1 function. This suggests that the RKIN1 protein has a role in the control of carbon metabolism in endosperms of rye.
一个编码假定的酵母（酿酒酵母）SNF1编码的蛋白丝氨酸/苏氨酸激酶同源物的cDNA，即cRKIN1，已从黑麦胚乳mRNA制备的文库中分离出来。Northern印迹分析表明，在发育中的胚乳中存在与cRKIN1相关的转录本，但在芽中不存在，Southern印迹分析表明存在一个小基因家族。SNF1在酵母的碳分解代谢物阻遏中起核心作用，并且酵母snf1突变体中RKIN1序列的表达恢复了SNF1功能。这表明RKIN1蛋白在黑麦胚乳的碳代谢控制中起作用。
